28_Cell Cycle I

Question 1

What does flow cytometry quantify?

Answer 1

Number cells as a function of DNA content per cell

Question 2

What phase of the cell cycle are the majority of cells in a culture in at a given time?
Which phase contains the least number of cells?

Answer 2

most: G1
least: S

Question 3

What is the result of fusing M-phase and S-phase cells?

Answer 3

chromosomal damage (pulverized DNA) due to compaction of actively dividing DNA.

Question 4

MPFs have 2 subunits; what are they?

Answer 4

CDK & a cyclin

Question 5

What is the effect of increasing cyclin concentration on CDKs?

Answer 5

they are activated => initiate entry into mitosis

Question 6

Which is degraded more, CKDs or cyclins?

Answer 6

cyclins

Question 7

What is the CDK in yeast?

Answer 7

cdc2 kinase – only has this one

Question 8

When is MPF activity the highest?

Answer 8

M-phase

Question 9

When is the concentration of mitotic cyclins the highest?

Answer 9

end of G2 (peaks just before initiation of M-phase)