27_Steroid hormones & gene regulation Flashcards
Which class of transcription factors have a ligand-binding domain?
nuclear hormone receptors
What is the function of the trans-activation domain on a TF?
recruitment of transcriptional partners/machinery
Which TF domain binds to DNA response elements?
DNA-binding domain
Name some examples of steroid hormone receptors.
glucocorticoid receptor, estrogen receptor, thyroid hormone receptor, retinoic acid receptors
Where do nuclear hormone receptors bind their ligand?
in the cytoplasm
What tag is used to mark genes according to parental origin?
DNA methylation
In DNA methylation, where is the methyl group added?
C5 position of cytosine residues
Where on the DNA does the GR bind?
GRE (glucocorticoid response element)
What is the chromatin remodelling complex recruited to acetylated histones? How does it affect chromatin?
SWI/SNF
It makes DNA accessible to RNA Pol/ other TF by either sliding the nucleosomes aside OR reorganizing them
Which TFs bind to the TATA box?
TBP
overall, the TFIID complex… which includes TBP and TAFII250, as well as other TFs
What does HDAC do?
HDAC = histone deacetylase
What is recruited to histones once deacetylated by HDAC?
Histone methyltransferases; K9 of H3 is methylated.
What are SMRT, N-CoR, and CoREST examples of?
transcriptional co-repressors
What is Sin3 an example of?
HDAC
What is SUV39H1 an example of?
Histone methyltransferase
