22 - NUCLEIC ACIDS Flashcards
why do we synthesize DNA and RNA molecules and their analogues?
how can human insulin be made from recombinant DNA technology
what is the phosphodiester method of synthesis (using condensing agents)
what is the first step of the phosphodiester method
what is step 2 of the phosphodiester method?
how does solid phase synthesis of DNA work?
what is the solid support used for DNA synthesis?
steps of solid phase synthesis of DNA
one connected to the insoluble material (sand)
the building blocks have building blocks to prevent polymerisation
activate the building blocks
couple on block to the other one
wash (filtration)
remove and repeat coupling
cleave the bond connecting it to sand to release the DNA strand
and then you can make the complementary strand
the ease of purification is a big advantage
easily automated and can be used for a small and large scale
how are nucleobases protected
how are the 3’ and 5’ OH protected?
cycle of solid phase synthesis
why is tetrazole used in the activating step and what is the activating + coupling step
what are common catalysts of the activation step
summary of the synthesis of nucleic acids
how is the DNA deprotected
overall yield vs coupling yield
how does automated solid phase synthesis work
what is the building block for RNA synthesis
how is the building block for RNA synthesis made
cycle of RNA synthesis
how are oligoribonucleotides deprotected?
what is mechanochemistry
synthesis of oligonucleotides by mechanochemistry
what is resonance acoustic mixing
