2.2 Cell Respiration Flashcards
What is ‘Respiration’?
A series of enzyme controlled reactions to breakdown glucose and release ATP
What is the word equation for respiration?
Glucose + Oxygen –> Carbon Dioxide + Water
What is the preferred respiratory substrate?
Glucose
What does ATP stand for?
Adenosine TriPhosphate
What are the two main roles of ATP?
- Act as energy transfer molecules
2. Carry out phosphorylation of molecules
What is ‘Phosphorylation’
Addition of an inorganic phosphate molecule
What is the reduced state of NAD?
NADH
Describe Reduction and Oxidation in terms of FAD
FAD - Oxidized State(loss of electrons)
FADH2- Reduced State(Gain of electrons)
What is the role of coenzymes in respiration?
Collect hydrogen ions and high energy electrons and deliver them to stage 3 of aerobic respiration
What is the role of dehydrogenase enzymes?
Remove hydrogen ions and high energy electrons during breakdown of glucose and pass them to coenzymes
What is the first stage of respiration?
Glycolysis
Where does Glycolysis occur?
Cytoplasm
What are the two stages known as in glycolysis?
Energy investment and Energy Payoff
What is meant by ‘Net gain of 2 ATP in Glycolysis’
- Invest 2 ATP
- Releases 4 ATP
Two more ATP that put in
What happens in animals after glycolysis in the absence of oxygen?
Pyruvate is converted into Lactic Acid
What is the end product of glycolysis?
Pyruvate
Is the conversion of pyruvate to lactic acid reversible or irreversible?
Reversible
What is pyruvate converted into in the absence of oxygen in yeast cells?
Ethanol and C02
What is energy from the breakdown of ATP used for?
Muscle Contraction
Repair of Cells
Transport
What is the optimum temperature for dehydrogenase enzymes?
37 degrees (human body temperature)
What is the second stage of aerobic respiration?
Citric Acid Cycle
Where does the Citric Acid Cycle Occur?
Matrix of Mitochondria
How many carbons does Pyruvate contain?
3 Carbons
What does the citric acid cycle result in?
- Generation of ATP
- Release of CO2
- Regeneration of Oxaloacetate
What happens to pyruvate before it becomes part of the cycle?
Converted into Acetyl Group, and then combines with coenzyme A to form Acetyl CoA
What is released in the conversion of pyruvate to Acetyl CoA?
CO2
NADH
How many carbons does Acetyl CoA contain?
2 Carbons
What does Acetyl CoA combine with to form citric acid?
Oxaloacetate
How many carbons does oxaloacetate contain?
4 carbons
At what two stages in the citric acid cycle is CO2 released?
During conversion of Pyruvate to Acetyl Group and during the conversion of Citric Acid to Oxaloacetate
What are the key products from stage 1 and 2 that are transported to stage 3?
NADH and FADH2
How many carbons does Citric Acid contain?
6 carbons
What do hydrogenase enzymes pass to coenzymes?
High energy electrons
Hydrogen Ions
What is the final stage in aerobic respiration?
Electron Transport Chain
Where does the electron transport chain take place?
Inner membrane folds of the mitochondria(Cristae)
**What is the final hydrogen/electron acceptor? **
OXYGEN
Why do we need to breathe in every few seconds (in relation to respiration)?
In order to provide oxygen as the final hydrogen/electron acceptor
Describe the steps in the electron transport chain?
- High energy electrons pump hydrogen ions across the membrane (provided by NADH and FADH2)
- Return flow of hydrogen ions generate ATP Synthase
- ATP synthase phosphorylates ADP to form ATP
- Oxygen bonds with hydrogen and electrons to form Water
Describe the roles of NADH and FADH2 in the electron transport chain?
NADH and FADH2 provide hydrogen ions and high energy electrons. The high energy electrons are used to pump the hydrogen ions across the membrane.
What can be used as an alternative respiratory substrate for Glucose?
Fructose and Maltose etc
Starch and Glycogen (Broken down forms)
What can be used as an alternative respiratory substrate for pyruvate?
Glycerol and Amino Acids
What can be used as an alternative respiratory substrate for acetyl CoA?
Fatty Acids and Amino Acids
What is the only type of molecule that can be an alternative respiratory substrate for an intermediate of the citric acid cycle?
Protein(Amino Acid)
What is released when amino acids are used as a respiratory substrate/
Urea
Name the two hydrogen carriers in aerobic respiration
NAD and FAD