21.4 Locating genes, genetic screening, and counselling Flashcards
1
Q
what are DNA probes?
A
short, single-stranded length of DNA with markers/labels added
2
Q
How do you make DNA probe
A
- make DNA comp. to base seq. of desired gene
- copy using PCR
- attach marker
3
Q
Main points for using DNA probes
A
- make DNA single stranded
- DNA probe mixed
- DNA probe attach
- wash unbound DNA probe
- use autoradiography/x-ray films/detect fluorescence
4
Q
How do you make the DNA double helix single stranded?
A
Heat it up to hydrolyse H bonds
5
Q
How do the DNA probes bind?
A
they bind by complementary base pairing
6
Q
why do we wash the unbound DNA probes?
A
to ensure that we’re not detecting the unbound DNA probes instead identifying the bound ones
