2013 NC State Mock Exam Tangent Questions E Flashcards
This organism was found in skin scrape of a sheep that was intensely pruritic. Name the organism.
Sarcoptes scabei
REPORTABLE. Note tarsus with unjointed stak and sucker. S. scabei Infests most mammalian species, but strains are host specific. Will infest humans but strains found on sheep and goats do not persist on people.
What is this device used for in rats?
Tail vein injection
1) http://www.plas-labs.com/illuminated_injection.cfm
2) PLAS LABS announcement. 2012. JAALAS. 51 (2): p. 739.
Domain 3; Primary species – Rat (Rattus norvegicus)
In a quarantine facility, what is the purpose of the pictured device?
To indicate that air flow is from area of least risk into the greatest hazard i.e. negative pressure
1) Hessler JR, Lehner NDM. Eds, 2009. Planning and Designing Research Animal Facilities. Academic Press, San Diego, CA. Chapter 26 – Quarantine Facilities and Operation
Domain 4;
Which of the following, measured by the equipment pictured, is one of the most common and abundant gaseous pollutants associated with the husbandry of research animals?
a. Carbon dioxide
b. Ammonia
c. Humidity
d. Nitrite
b. Ammonia
1) Hessler JR and Lehner NDM, eds. 2009. Planning and Designing Research Animal Facilities, 1st ed. Academic Press: San Diego, CA. Chapter 7 – Environmental Considerations for Research Animals, p. 63.
2) National Research Council (U.S.). 2011. Guide for the Care and Use of Laboratory Animals. Washington, D.C.: National Academies Press. Chapter 3- Environment, Housing and Management, p. 71.
Domain 4; Animal Care
The photomicrograph below is from a dead mouse. What is the most likely disease? What immune component is an absolute requirement for recovery from this disease?
Syncytium is pictured. Therefore, disease is most likely MHV as syncytium formation is a hallmark of MHV infection in many tissues. Commonly found at the margin of necrosis.
Functional T cells are required for recovery from the enterotropic form of MHV.
1) Percy DH and Barthold SW. 2007. Pathology of Laboratory Rodents and Rabbits, 3rd edition. Blackwell Publishing: Ames, Iowa. Chapter 1 – Mouse, p. 32.
2) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 1 – Biology and Diseases of Mice, p. 75.
3) http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/171544.htm
Domain 1; Primary Species – Mouse (Mus musculus)
The pictured piece of equipment is used for evaluating what in mice and rats?
Elevated Plus Maze; Evaluating anxiety paradigms
1) Costa R, Tamascia ML, Nogueira MD, Casarini DE, Marcondes FK. 2012. Handling of Adolescent Rats Improves Learning and Memory and Decreases Anxiety. J Am Assoc Lab Anim Sci 51:548-553
2) Zimberknopf E, Xavier GF, Kinsley CH, Felicio LF. 2011. Prior Parity Positively Regulates Learning and Memory in Young and Middle-Aged Rats. Comp Med 61:366-377
Domain 3; Primary Species – Rat (Rattus norvegicus) and Mouse (Mus musculus)
Which of the following methods of euthanasia is conditionally acceptable in the laboratory animal species shown below?
a. Benzocaine hydrochloride
b. Double pithing
c. Decapitation and pithing
d. Hypothermia
c. Decapitation and pithing
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 17- Biology and Diseases of Amphibians, p 814.
2) AVMA Guidelines on Euthanasia. June 2007. Available at: http://www.avma.org/issues/animal_welfare/euthanasia.pdf. Appendix 1- Agents and Methods of Euthanasia by Species, p 28.
3) (Image source: http://www.uniprot.org/taxonomy/8355)
Domain 2; Secondary species – African clawed frog (Xenopus spp.)
A 12 wk old rabbit exhibits profuse watery diarrhea and dies 2 days after exhibiting clinical signs. Gross necropsy reveals thickened, edematous bowel wall with patches of hemorrhaging and has multifocal pinpoint white foci along the liver parenchyma and heart. Warthrin-Starry silver stain of the liver reveals:
What is the most likely etiology?
Clostridium piliforme
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 9 – Biology and Diseases of Rabbits, p. 340-341.
2) Porter RS 2011. The Merck Veterinary Manual (19th ed., Overview of Tyzzers Disease). Retrieved December 12, 2012.
Domain 1; Primary Species – Rabbit (Oryctololagus cuniculus)
The image above represents a method of:
a. Euthanasia of Salmonids
b. Euthanasia of Xenopus laevis
c. Euthanasia of Xenopus
d. Euthansia of Danio rerio
d. Euthansia of Danio rerio
1) M Wilson, Jolaine; M Bunte, Ralph; J Carty, Anthony. 2009. Evaluation of Rapid Cooling and Tricaine Methanesulfonate (MS222) as Methods of Euthanasia in Zebrafish (Danio rerio) JAALAS 48(6): pp. 785-789
Domain 2. Secondary species – Zebrafish (Danio rerio)
What is this device used for?
Nonhuman primate enrichment
Reference: http://www.primateproducts.com/enrichment-devices/puzzle-feeder/
Domain 4;
This image depicts what piece of equipment that may be found in an animal facility?
RFID tag
1) Guide for the Care and Use of Laboratory Animals. Institute of Laboratory Animal Resources, National Research Council, National Academy of Sciences. National Academy Press, Washington, DC, 2011. P. 75.
2) http://www.allentowninc.com/en/products/wicom/sensus.aspx
Domain 4
A 5 week old guinea pig presents with conjunctivitis with serous to purulent discharge. A conjunctival smear stained with Giemsa is shown. What is the most likely diagnosis, and how should it be treated?
Chlamydophila caviae (Guinea Pig Inclusion Conjunctivitis - GPIC)
Self-limiting disease and can additionally include rhinitis, genital infections, lower respiratory infections, and abortions. Can be treated with sulfonamides. Does not affect humans, but has been used as a model for human chlamydophila infection.
1) Percy, DH, Barthold, SW. 2001. Pathology of Laboratory Rodents and Rabbits. 3rd edition. Iowa State Press: Ames, IA. Chapter 5 – Guinea Pig, p.228
2) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 6 – Biology and Diseases of Guinea Pigs, p. 220-221.
Domain 1; Secondary Species – Guinea Pig (Cavia porcellus)
The pink arrow is pointing to what structure?
peg teeth
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 9 – Biology and Diseases of Rabbits, p. 331.
2) Sukow MA, Stevens KA, Wilson RP, eds. 2012. The Laboratory Rabbit, Guinea Pig, Hamster and Miscellaneous Rodents. Academic Press, San Diego, CA. Chapter 8 – Anatomy, Physiology, and Behavior, pp. 199.
Domain 1; Primary Species – Rabbit (Oryctolagus cuniculus)
What is the genus and species of this parasite found in a Syrian hamster?
Demodex criceti
Demodectic mites are still prevalent at about 5%. D. aurati also infest hamsters, but have a characteristic thin, elongate body and a sharp opisthotomata at the posterior end.
1) Baker, DG. 2007. Flynn’s Parasites of Laboratory Animals. 2nd edition. Blackwell Publishing: Ames, IA. Chapter 12 – Parasites Of Hamsters, p. 404-405
2) Suckow MA, Stevens KA, Wilson RP, eds. 2012. The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, 1st edition. Academic Press: San Diego, CA. Chapter 5 – Biology And Diseases of Hamsters, p. 857-859.
Domain 1; Secondary species – Syrian Hamster (Mesocricetus auratus)
Tissue from the distal small intestine of a young mouse. Name the etiologic agent.
Mouse Adenovirus-2
Intranuclear adenoviral inclusions in intestinal epithelium are pathognomonic and differentiate MAdV-2 infection from other viral infections in mice.
1) Percy DH and Barthold SW. 2007. Pathology of Laboratory Rodents and Rabbits, 3rd edition. Blackwell Publishing: Ames, Iowa. Chapter 1 – Mouse, p. 18.
2) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 3 – Biology and Diseases of Mice, p. 64.
Domain 1; Primary Species – Mouse (Mus Musculus)
This picture depicts a common injury associated with incorrect handling and restraint of this species. Where is the lesion most likely located?
L7
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 9 – Biology and Diseases of Rabbits, pg. 352
2) Hrapkiewicz K, Medina L. 2010. Clinical Laboratory Animal Medicine – An Introduction, 3rd edition. Blackwell Publishing: Ames, Iowa. Chapter 10 – Rabbits, pg 205.
Domain 4; Primary Species – Rabbit (Oryctolagus cuniculus)
This T-cell deficient GEM mouse presented with respiratory distress. This finding would lead you to suspect what pathogen and what other staining technique would be useful in confirming your diagnosis?
Pneumocystis murina; silver-based or PAS staining
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 3 – Biology and Diseases of Mice, p. 97.
2) Percy DH and Barthold SW. 2007. Pathology of Laboratory Rodents and Rabbits, 3rd edition. Blackwell Publishing: Ames, Iowa. Chapter 1 – Mouse, p. 84.
Domain 1; Primary Species – Mouse (Mus musculus)
The image of the laboratory animal species shown below depicts which procedure that is commonly performed prior to parturition to minimize fetal contamination during the birthing process?
a. Flagging
b. Crutching
c. Processing
d. Grafting
b. Crutching
References: (Image source: http://www.sheep101.info/201/shearing.html)
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 14- Biology and Diseases of Ruminants: Sheep, Goats, and Cattle, p 531.
2) Federation of Animal Science Societies, FASS 2010. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (Ag Guide), 3rd edition. Available at http://www.fass.org. Chapter 10- Sheep and Goats, p 134.
Domain 4; Secondary species- Sheep (Ovis aries)
This animal is part of an R01 study. If this animal shown in the picture is housed in a laboratory for 16 hours. Does the laboratory need to be inspected during IACUC semi-annual inspections, and why or why not?
No, PHS requires inspections of all facilities were animals are housed for more than 24 hours.
1) Public Health Service on Humane Care and Use of Laboratory Animals, Office of Laboratory Animal Welfare, National Institutes of Health, pg 8. Revised August 2002
2) http://www.aphis.usda.gov/animal_welfare/2011_Inspection_Guide/9.8.5%20Facility%20Inspection.pdf
Domain 5; Tertiary Species – Other birds
Use of the above vector in vertebrate animals at an institution that receives NIH funds, would require the institution to follow what set of federal rules:
NIH Guidelines for Research Involving Recombinant DNA Molecules
References:
http://oba.od.nih.gov/rdna/nih_guidelines_oba.html
Domain 5.
What is the pictured device used for?
Euthanasia
References: Valentine H., et. Al. 2012. Sedation or inhalant anesthesia before euthanasia with CO2 does not reduce behavior or physiologic signs and stress in mice. Vol. 51, No. 1, p. 50-57.
Domain 2; Primary species – Mice (Mus musculus)
The compound shown can be used to remove which molecule from water used to house amphibians?
Chlorine
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd
edition. Academic Press: San Diego, CA. Chapter 17 – Biology and Diseases of Amphibians, p.
797.
2) Browne RK, Odum RA, Herman T, Zippel K. 2007. Facility Design and Associated Services for the
Study of Amphibians. ILAR J 48: 188-202.
Domain 4; Tertiary Species – Other Amphibians
Interest in this species has centered around its susceptibility to a variety of diseases including hantaviruses and Lyme disease. What is this animal?
Peromyscus
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 7 – Biology and Diseases of Other Rodents, p. 266-267.
2) Suckow, Mark A., Stevens, Karla A., Wilson, Ronald P., eds. 2012. The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents Elsevier: San Diego, CA., Chapter 46 – Other Rodents, Deer Mice, White-Footed Mice, and their Relatives. Pg 1082-1083.
Domain 3 – Tertiary species – Other Rodents
Which of the following statements is true regarding the image above?
a. Only the government may purchase dogs for research from this source.
b. Any institution may purchase dogs for research from this source.
c. This is not an acceptable source for research dogs.
d. This is a conditionally acceptable source for research dogs.
b. Any institution may purchase dogs for research from this source.
1.) The Animal Welfare Act 9 CFR, Part 2- Regulations, Subpart A- Licensing, §2.1 Requirements and Application (11-01-05 Edition, p.26).
2.) Guide for the Care and Use of Laboratory Animals, 8th edition. 2011. National Research Council: Washington, DC 2011. Chapter 2- Animal Care and Use Program, p. 12.
Domain 5; Primary Species – Dog (Canis familiaris)
The following species should be incubated at 99.5 Fahrenheit degrees for how many days to induce hatching?
21 days
1)http://www.backyardchickens.com/a/how-to-incubate-hatch-eggs-just-21-days-from-egg-to-chicken
2)http://www.aaalac.org/accreditation/faq_landing.cfm#C2
Domain 4; Tertiary species – chickens (Gallus domesticus)
What grimace scale score do each picture represent?
A = 0 (No pain)
B = 1 (Moderate)
C = 2 (Obvious)
1.) Matsumiya LC, Sorge RE, Sotocinal SG, Tabaka JM, Wieskopf JS, Zaloum A, King OD, Mogil JS, Using the Mouse Grimace Scale to Reevaluate the Efficacy of Postoperative Analgesics in Laboratory Mice. J Am Assoc Lab Anim Sci. 2012. 51(1):42-49.
2.) Langford DJ, Bailey AL, Chanda ML, Clarke SE, Drummond TE, Echols S, Glick S, Ingrao J, Klassen-Ross T, Lacroix-Fralish ML, Matsumiya L, Sorge RE, Sotocinal SG, Tabaka JM, Wong D, van den Maagdenberg AM, Ferrari MD, Craig KD, Mogil JS. Coding of facial expressions of pain in the laboratory mouse. Nat Methods. 2010 Jun;7(6):447-9.
Domain 2; Primary Species – Mice (Mus musculus)
The equipment shown above is utilized primarily to
Sanitize shoe-box rodent cages, cage pans, water bottles and small equipment
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd ed. Academic Press, San Diego, CA. Chapter 21, p. 933.
2) http://www.labrepco.com/store/categories/view/id/223/category/Tunnel_Washer
Domain 4;
What are the typical clinical signs in mice with this organism?
Helicobacter spp.
Usually asymptomatic in immunocompetent mice. Most, perhaps all, Helicobacter spp. are capable of inducing proliferative typhlocolitis in immunodeficient mice.
A/JCr, SCID/NCr, BALB/cANCr, C3H/HeNCr, and SJL/NCr are susceptible to hepatitis, wheras B6 and B6C3F1 are hepatitis-resistant.
A/J mice also develop earlier onset and higher prevalence of hepatocellular tunnors when infected with H. hepaticus. More common in males, and incidence increased in ages > 6 months.
What is the most likely diagnosis?
Zymbal’s gland adenocarcinoma
Can be adenomas or adenocarcinomas. Malignant tumors that are locally invasive but not metastatic.
1) Percy DH and Barthold SW. 2007. Pathology of Laboratory Rodents and Rabbits, 3rd edition. Blackwell Publishing Ltd: Ames, Iowa. Chapter 2 – Rat, p. 175.
2) Rudmann D, Cardiff R, Chouinard L, et al. Proliferative and Nonproliferative lesions of the Rat and Mouse Marray, Zymbal’s. Preputial, and Clitoral Glands. Toxicologic Pathology, 40:7S,1533-1601, 2012.
Domain 1; Primary Species – Rat (Rattus norvegicus)
What is the most likely etiology of the condition shown below?
- a. Sarcoptes scabeii
b. Psoroptes cuniculi
c. Cheyletiella parasitovorax
d. Passalurus ambiguous
e. Notoedres cati*
b. Psoroptes cuniculi
All stages of the mite (egg, ;arva, protonymph, adult) occur on thre host. Feed on sloughed skin cells, lipids, serum, hemoglobin, rbc. Entire life cycle is 21 days. Relatively resistant to drying and temperature, and can survive off host for 7-20 days in temperatures 5-30 C and 20-75% humidity.
1) Fox JG, Anderson LC, Loew FM, Quimby FW, eds. 2002. Laboratory Animal Medicine, 2nd edition. Academic Press: San Diego, CA. Chapter 9 Rabbits p349.
2) Percy DH and Barthold SW. 2007. Pathology of Laboratory rodents and rabbits, 3rd edition. Blackwell Publishing: Ames, IA.
3) Photo credit http://www.foxyrabbits.eu/teadmistepagas/haigused/lest/?lang=en
Domain 1; Primary species – Rabbit (Oryctolagus cuniculus)