2/14 JPR - Lu & Era

Question 1

What is the connection between periodontal disease and chronic kidney disease? - Umbrella review

He et al JPR

Answer 1

Umbrella review
n=18 studies

Chronic kidney disease (CKD) defined by:
* One or more markers of kidney damage
* decreased GFR <60 mL/min / 1.73 m2
* Or, both of the above (at least 3 months’ duration)

Results:
“A bi-directional relationship exists between CKD and periodontitis;” however, Weak evidence demonstrating NSPT improves kidney functions.

Lots of heterogeneity in included studies

Question 2

What is the in vitro study into 3 different PRF preparations for osteogenesis potential?

Kosmidis et al. JPR

Answer 2

• L-PRF: Leukocyte platelet-rich fibrin: the “standard” PRF.
• A-PRF: Advanced Platelet-rich fibrin. Generated with a lower RCF (rotary centrifugal force). It has a porous fibrin structure which traps platelets and leukocytes
• i-PRF: injfectable PRF: even lower RCF; obtains a liquid material which can be used alone or mixed with other grafts before clotting.

Study aims to compare these 3 PRF’s for osteogenesis in vitro

6 male patients - collected samples and pre-cultured for 6 days. Seeded osteoblasts and cultured either with conditioned medium from the 3 PRF’s, or with regular culture medium.

Evaluated osteogenic capacity of cells at 0, 7, 14, 21, 28 days.

RESULTS:
* RUNX-2 indicates higher number of mature osteoblasts in A-PRF and L-PRF.
*However, A-PRF and L-PRF may slow down the calcification process.
* i-PRF increases activity of alkaline phosphatase = promotes differentiation of the osteoblasts.

Question 2

Cross-sectional study

Describe the study oxidative stress markers in the gingival crevicular fluid of pregnant women with gestational diabetes, vs. healthy pregnant women

Answer 2

Results:
SSD in oxidative stress marker levels (TAS/TOS) in the pregnant women with gestational diabetes.

Question 2

Is there an association between sickle cell disease and periodontal disease? - systematic review

Batista da Silva et al. JPR

Answer 2

Systematic review
n= 13 (for qualitative report), but n = 9 for quantitative analysis

Results:
Gingival index was significantly higher in sickle cell patients.

However: No association between sickle cell disease and periodontitis.

Question 2

What is the association between androgenetic alopecia and periodontitis?

Karasu et al JPR

Answer 2

Cross-sectional study
n= 385

Prevalence by age of androgenetic aloplecia:
* 25-34 yrs: 25%
* 35-44 yrs: 74%
* 45-54 yrs: 92%
* 55-65 yrs : 91%

Results:
SSD association with aloplecia and periodontitis the 25-34 and the 35-44 year group.

Note: Oral hygiene and smoking were not assessed

Question 3

Describe how HbA1c is affected by amoxicillin & metronodazole as an adjunct to SRP

Xu et al JPR

Answer 3

• 26 patients in test group (SPT + 500 mg metronidazole + 500 mg amoxicillin TID for 7 days
• 23 patients in control: SPT only

3 mo followup

RESULTS: NSSD in HbA1c between the groups.