15. Immune System Flashcards
What is the immune system?
A versatile defence system that protects us from pathogenic microbes
What are the layers of the defence strategy?
- First line - innate immunity
- Second line - innate immunity
- Third line - specific/adaptive immunity
What is the first line of defence - innate immunity?
Physical barrier created by the skin and mucous membranes
What is the second line of defence - innate immunity?
Non-specific immune response that includes some immune cells, proteins, fever and inflammation
What is the third line of defence - specific/adaptive immunity?
Activated by the innate immune system, producing a response towards a specific pathogen
What is a pathogen?
An infectious agent that can cause disease in a host
How can pathogens enter the body?
Breaks in the skin Respiratory system Digestive system Reproductive system (male and female) Eyes
What is an antigen?
A substance that can be recognised by leukocytes
What are the two types of antigen?
- Foreign antigens
2. Self-antigens
What are foreign antigens?
Microbes
Food
Drugs
What are self-antigens?
Found on cell membranes
What do antigens usually consist of?
Proteins (specific 3D shape)
What are antibodies?
Proteins produced in response to a specific antigen
What do antibodies and the specific antigens do?
Combine
What makes the skin a first line of defence against pathogens?
Made up of layers of tightly packed epithelial cells
Outer epidermis - consists of dead epithelial cells which sheds every 40 days to remove microbes
Dermis - contains accessory structures such as sebaceous glands and sweat glands
How does sweat perform an immune function?
Removes microbes from skin
Contains IgA
How does sebum perform an immune function?
Contains fatty acids which inhibit microbial growth
Where can mucous membranes be found?
Digestive tract
Respiratory tract
Urogenital tract
Conjunctiva
What is the function of mucous membranes?
Prevent entry into the membrane
How do the mucous membranes perform an immune function?
Mucous traps microbes and foreign particles
Saliva, tears and mucous secretions wash away the trapped microbes
They also contain anti-microbial substances
How does the immune function work in the respiratory tract?
Mucocillary escalator
Cilia propel any foreign substances towards the pharynx where they are swallowed or coughed up
How do tears and saliva perform an immune function?
Contain IgA and lyzozymes
Lyzozymes are enzymes that break down bacterial cell walls
How does the nose perform an immune function?
Hairs in the nose filter air
How does the vagina perform an immune function?
In menstruating women, vagina is acidic making it unfavourable for microbes
How does gastric acid perform an immune function?
Acidity destroys many bacteria
How do microflora perform an immune function?
Outcompete pathogens for attachment sites on epithelial cell surfaces (e.g. GALT)
How does the excretion of urine and faeces perform an immune function?
Both expel microbes
How does vomiting and diarrhoea perform an immune function?
Both rapid means of expelling pathogens
When does the second line of defence get activated?
When pathogens penetrate the physical and chemical barriers of the skin and mucous membranes
What does the second line of defence consist of?
Transferrins Complement system Phagocytes Natural killer cells Inflammation Cytokines Fever
What are transferrins?
Iron-binding proteins in blood
How do transferrins work?
Inhibit the growth of certain bacteria by reducing the amount of available iron
Why are transferrins necessary?
Without transferrins, bacteria would use the iron available for their growth
What is the complement system?
Secondary defence system made up of over 30 proteins produced by the liver
How are complement proteins identified?
By a letter (mostly C) and a number e.g. C3
How does a complement protein start out?
Inactive
Only become active when split by enzymes into active fragments e.g. C3 = C3a and C3b
What happens when a complement protein is activated
Acts in a cascade
What is the most common mechanism through which complement proteins are activated?
Classical pathway
What is the ‘classical pathway’?
Where antigens and antibodies join together
What is the role of complement proteins?
- Promoting phagocytosis
- Contributing to inflammation
- Causing cytolysis
How do complement proteins promote phagocytosis?
The fragment C3b coats a microbe in a process called opsonisation
This promotes the attachment of a phagocyte to a microbe
How do complement proteins contribute to inflammation?
C3a and C5a bind to mast cells and cause them to release histamine
How do complement proteins cause cytolysis?
The final complement proteins join together and create a hole in the foreign cell
The hole fills with tissue fluid and makes the cell swell and rupture
What are cytokines?
Small protein hormones that stimulate or inhibit normal cell functions
Mediate the connection between the innate immune system and the adaptive immune system
What are cytokines secreted by?
Leukocytes
What are the types of cytokines?
Interleukins
Interferons
Tumour necrosis factor
What are interleukins?
Mostly produced by T-helper cells
Mediators between leukocytes
What are interferons?
Anti-viral proteins produced by cells that are infected by a virus
What is tumour necrosis factor?
Promotes the accumulation of neutrophils and macrophages to an area
Causes cell death
How do interferons work?
They diffuse to uninfected neighbouring cells and induce synthesis of anti-viral proteins that interfere with viral replication
Do interferons stop a virus attaching to it?
No but they prevent the virus from replicating
What do phagocytes do?
They perform phagocytosis - cell digestion - of anything they don’t recognise
Non-selective
How do phagocytes work?
They attach to sites of inflammation by chemotaxis
What is chemotaxis?
Directed migration of a cell in response to a chemical stimulus
What are the two major types of phagocyte?
Neutrophils
Macrophages
What do neutrophils do?
First to migrate to a scene of inflammation
Like neighbourhood watch
Try to mop up the day to day problems
What do macrophages do?
They follow the neutrophils and eat up the remnants of whatever needs to be broken down and digested
They engulf and digest the pathogen, excrete some of the waste and display the rest on the cell membrane to present to T-lymphocytes
Like the police coming to the scene of a crime
Where can macrophages be found?
In tissue
Where can monocytes be found?
In blood
What do monocytes do?
Migrate to the site of infection and enlarge to form wandering macrophages
What do fixed macrophages do?
Stand guard in specific tissues
In which tissues can fixed macrophages be found?
Histiocytes (connective tissue macrophages)
Kupffer cells - liver
Microglia - nervous tissue
Alveolar - lungs
Langerhans cells - skin
Tissue macrophages - spleen, bone marrow, lymph nodes
What are the stages of phagocytosis?
- Chemotaxis - release of chemicals by leukocytes, damaged tissue and activated complement that attract phagocytes
- Adherence - attachment of phagocyte to target - aided by complement
- Ingestion - cell membrane extends to engulf the microbe
- Digestion - ingested structure merges with lysozymes to be digested
- Excretion - ingested material is excreted
What are natural killer cells?
Non-specific lymphocytes
What percentage of lymphocytes do natural killer cells make up?
5-10%
Where can natural killer cells be found?
Blood
Lymph nodes
Spleen
Bone marrow
How do natural killer cells work?
They attack anything that they don’t recognise including abnormal body cells e.g. cancerous cells
They bind to a target cell and release granules containing the protein perforin
What does perforin do?
It inserts into the cell membrane and creates a channel for tissue fluid to flow into the cell - cytolysis
What is inflammation?
A non-specific defensive response to tissue damage
What can inflammation be caused by?
Pathogens Abrasions Chemicals Cell distortion/disturbance Extreme temperatures
What response does inflammation create?
Non-specific
What are the cardinal signs of inflammation?
Redness Heat Pain Swelling Loss of function
What are the stages of inflammation?
- Vasodilation and increased permeability
- Emigration of phagocytes
- Tissue repair
How does vasodilation help inflammation?
Allows additional blood, oxygen, nutrients, immune cells to get to the area
Toxins and dead cells are removed
How does increased permeability help inflammation?
Permits the movement of immune cells, defensive proteins (antibodies) and clotting factors into the tissue
How do vasodilation and increased permeability work together to help inflammation?
Together they create redness, swelling and heat
What is the pain of inflammation a result of?
Injury to neurons and toxic chemicals released by microbes
What happens when phagocytes migrate to an area?
- Phagocytes migrate to the scene (via chemotaxis)
- Neutrophils stick to the endothelium during vasodilation and squeeze through the vessel wall to reach the damaged area
- Monocytes quickly follow and transform into wandering macrophages
- Dead phagocytes accumulate pus
What are the types of inflammatory mediators?
Histamine
Leukotrienes
Kinins
Prostaglandins
What is histamine and its function?
Released by mast cells and basophils
Causes vasodilation and increased permeability
What are leukotrienes and their function?
Released by basophils and mast cells
They attract phagocytes and increase permeability
What are kinins and their function?
Proteins
Causes vasodilation and increased permeability
Also attract phagocytes and induce pain
What are prostaglandins and their function?
Lipids released by damaged cells
Enhance effects of histamine and kinins (intensifying the pain)
What are the benefits of inflammation?
Promotes phagocytosis through increased temperature
Promotes immune response via vasodilation/permeability
Dilutes toxins
Fibrin formation - isolates affected area and helps bind wound edges
What are the harmful effects of inflammation?
Swelling - dangerous if in cranium
Pain - can become chronic
Adhesions and scar tissue
Atherosclerosis
What are the outcomes of inflammation?
Resolution
Chronic inflammation
Granuloma
Fibrosis
What is chronic inflammation?
If the injury-causing agent persists
Can cause chronic pain
Prevalence of macrophages, plasma cells and lymphocytes