12.11-12.16 Reading Quiz Flashcards
How do you prove two samples come from the same person?
Matching genetic markers
DNA profiling
The analysis of DNA samples to determine whether they came from the same individual
Polymerase chain reaction
Technique that can amplify a specific segment of DNA; repeated three step cycle
Primers in PCR
Short, chemically synthesized single-stranded DNA molecules with sequences that are complementary to the sequences of the target sequence
Three steps of PCR
- The PCR reaction mixture is heated
- Then the mixture is cooked to an optimal temp
- At the heat stable temp the DNA replicates
Short tandem repeat (STR)
Relevant type of repetitive DNA consisting of short sequences repeating many times in a row
Single nucleotide polymorphism
Single-base-pair variation in the genome; occur once in every 100 to 300 base pairs
Restriction fragment length polymorphism
When a single nucleotide polymorphism alters a restriction site and the sequence is recognized by a restriction enzyme