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Immunosero Lec (Midterm) > 1.2 Precipitation and Agglutination Reactions part 2 > Flashcards

1.2 Precipitation and Agglutination Reactions part 2 Flashcards

1
Q

Detects unknown antibodies in patirent’s serum using known antigens bound to a particulate

A

Passive agglutination

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2
Q

What is the unknown in passive agglutination?

A

Antibodies

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3
Q

Detects unknown antigens in the patient’s serum using known antibodies bound to particulate carrier

A

Reverse passive agglutination

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4
Q

What is the unknown in reverse passive agglutination?

A

Antigen

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5
Q

generally used in the blood bank for RBC phenotyping and antibody screening

A

Anti-human Globulin test / Coomb’s test

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6
Q

The AHG attach to what part of another antibody?

A

Fc portion

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7
Q

What type of immunoglobulin is AHG?

A

anti-IgG

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8
Q

Detects in VIVO sensitization of RBC

A

Direct AHG test

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9
Q

This test is used to detect autoimmune RBC?

A

Direct AHG test

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10
Q

Detects in Vitro sensitization of RBC

A

Indirect AHG test

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11
Q

AHG test that is used in cross matching or antibody screening identification

A

Indirect AHG test

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12
Q

What is the specimen for indirect AHG test?

A

Patient serum

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13
Q

What is the specimen for direct AHG test?

A

Patient RBCs

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14
Q

Based on competition between particulate and soluble antigens for limited antibody-combining sites

A

Agglutination inhibition

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15
Q

This test has Two-step procedure?

A

Agglutination inhibition

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16
Q

The first step in agglutination inhibition is not visible?

A

T

17
Q

What is the positive result of agglutination inhibition?

A

No agglutination

18
Q

Uses the same principle except that RBCs are used as indicator particles

Used to detect antibodies to certain viruses such as rubella, influenza and RSV

A

Hemagglutination inhibition

19
Q

What is the positive result for Hemagglutination inhibition?

A

No agglutination

20
Q

What is the indicator particle used in hemagglutination inhibition?

A

RBCs

21
Q

What is the indicator particle used in hemagglutination inhibition?

A

RBCs

22
Q

Based on the principle that antigen-antibody complexes bind or fix the complement and are unable to react with sensitized indicator cells

A

Complement fixation

23
Q

What immuoglobulin is applicable only in complement fixation?

A

IgM

24
Q

What is the positive result for complement fixation?

A

No hemolysis

because complement attach to antibody (This prevents attachment to RBC to avoid hemolysis)

25
Q

Complement fixation is used to detect Late stages of diseases?

A

False, EARLY stages

26
Q

What is the indicator for Complement fixation?

A

RBC

Immunosero Lec (Midterm) (10 decks)

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