10. Biotechnology

Question 1

PCR

Answer 1

polymerase chain reaction

Question 2

can PCR happen outside the cell?

Answer 2

yes, it can happen in buffers/proveza

Question 3

which polymerase is used in PCR?

Answer 3

TAQ-polymerase

Question 4

the 1st step of PCR is

Answer 4

denaturation

Question 5

the required temperature during denaturation is

Answer 5

95 g.celcius

Question 6

the 2nd step of PCR is

Answer 6

Annealing

Question 7

the required temperature during annealing is

Answer 7

50-60 g.celcius

Question 8

why is the temp. lowered during the annealing/binding phase?

Answer 8

to allow single-stranded DNA primers to bind to the complementary sequences of DNA

Question 9

the 3d phase of PCR is

Answer 9

Extension

Question 10

at what temperature does TAQ-polymerase work?

Answer 10

72 g.celcius

Question 11

what happens during the extension phase?

Answer 11

TAQ-polymerase synthesizes new DNA strands by using free nucleotides, using primers as starting points

Question 12

what are restriction enzymes called?

Answer 12

restriction endonucleases

Question 13

where are adult stem cells found?

Answer 13

bone marrow
brain
skin
hair
eyes
dental pulp

Question 14

can stem cells differentiate into eggs and sperm?

Answer 14

yes, only embryonic stem cells

Question 15

what kind of cells are embryonic stem cells?

Answer 15

pluripotent