01 LAB: Methods of Blood Collection

Question 1

The methods of blood collection was published by ___

Answer 1

Clinical & Laboratory Standard Institute (CLSI)

Question 2

3 methods of blood collection

Answer 2

Skin puncture
Venipuncture
Arterial blood puncture

Question 3

Effect of posture in blood sample:

Increase in ___, ___, ___

Answer 3

Protein
Cholesterol
Iron

Question 4

Analytes increased in the morning and decreased in the afternoon (3)

Answer 4

Cortisol
TSH
Iron

Question 5

Analyte decreased in the morning and increased in the afternoon

Answer 5

Eosinophil count

Question 6

Exercise increases which analyte? (7)

Answer 6

Creatinine
TP
Creatine kinase
Myoglobin
AST
WBC count
HDL

Question 7

Effect of stress in the WBC count

Answer 7

Temporary increase (in children)

Question 8

Diet affects which analytes? (2)

Answer 8

Blood glucose
Lipid content

Question 9

Smoking increases ___ and ___, and decreases ___

Answer 9

WBC count, hemoglobin levels

Pulmonary function

Question 10

Best site of collecting capillary blood

Answer 10

Earlobe

Question 11

Skin puncture:

The puncture is ideally ___ in depth

Answer 11

2-3 mm

Question 12

Skin puncture:

Difficulty in obtaining blood can be relived by using ___ ___ onto the site

Answer 12

Warm compress

Question 13

Order of draw of microtainer

Answer 13

Lavender&raquo_space; Green&raquo_space; Mint Green&raquo_space; Gray&raquo_space; Gold&raquo_space; Red

Question 14

Venipuncture:

Tourniquet is applied ___ above the site of puncture

Answer 14

3-4 inches

Question 15

Venipuncture:

The gauge of the needle is usually ___

Answer 15

20-21

Question 16

Venipuncture:

The syringe should be inserted at ___ angle and a depth of about ___

Answer 16

15 degrees
0.5-1 cm

Question 17

Anticoagulant that inactivates factor Xa and acts as antithrombin

Answer 17

Heparin

Question 18

Amount of anticoagulant in green top tube

Answer 18

14-17 micoliter per mL of blood

Question 19

Action of EDTA in the blood

Answer 19

Chelates calcium

Question 20

Ratio of EDTA anticoagulant to blood in lavender top tube

Answer 20

1:9

Question 21

Percentage of anticoagulant in a blue top tube

Answer 21

3.2%