01 LAB: Blood Smear Preparation Flashcards
Complete blood count is also called ___
Hemogram
The peripheral blood film is preferably prepared within ___
2-3 hours
Simplest and most common method of creating blood films
Two-slide/ Wedge method
Wedge technique:
Size of blood: ___
Angle of spreader slide: ___
2-3 mm
30-45 degrees
An older technique that is now used rarely for PBS, but is sometimes still used for making bone marrow aspirate smears
Two-cover slip/ Elrich’s two-coverglass method
This smearing technique produces an excellent leukocyte distribution, which lends itself to more accurate determination of differential counts
Two-cover slip/ Elrich’s two-coverglass method
This smearing technique produces uniform blood film, monolayer of cells distributed accurately
Spinner method
This automated smear preparation allows the production of blood smear for every 30 seconds
Sysmex SP-1000i
This automated smear preparation allows the creation of four slides from a 90 microliters of sample and 12 slides from a single sample presentation
DxH Slidemaker Stainer II Cellular Analysis System
The specific anticoagulant used to observe cell morphology
Ethylenediaminetetraacetic acid (EDTA)
Stain used for the preparation of blood smear
Romanowsky stain
Eosin and methylene blue stains are ___ stains
Polychrome
Anticoagulant NOT used for peripheral blood smears:
Causes blue background
Heparin
Anticoagulant NOT used for peripheral blood smears:
Distort cellular morphology
Oxalate and Fluoride
Give the reagent and how much time needed:
Used to fix the blood to the slide
Methanol
30 seconds
Give the reagent and how much time needed:
Stains basic components such as hemoglobin or eosinophilic granules
Eosin stain
6 seconds
Give the reagent and how much time needed:
Stains acidic components such as RNA
Methylene blue
4 seconds
Component of buffer solution needed for PBS
0.05 M sodium phosphate or aged distilled water
pH level of the buffer solution needed for PBS
6.4
Give the stain color in PBS:
RBC
pink to salmon
Give the stain color in PBS:
Nuclei
Dark blue to purple
Give the stain color in PBS:
Cytoplasmic granules of neutrophils
Lavender to lilac
Give the stain color in PBS:
Cytoplasmic granules of basophils
Dark blue to black
Give the stain color in PBS:
Cytoplasmic granules of eosinophils
Red to orange
Identify the magnification to use:
Validates the overall quality of the film including abnormal distribution of RBCs
10x
Identify the magnification to use:
Rapid detection of large abnormal cells
10x
Identify the magnification to use:
Quality control tool for validating WBC
40x or 50x
Identify the magnification to use:
Used for WBC differential count
100x
