02 LAB: RED BLOOD CELL COUNT Flashcards
RBC Count is the number of RBCs in ___ of blood
1 L
In traditional units, RBC count is measured by ___
number of cells per cubic millimeter
Preferred tube for RBC count
EDTA
Characteristics of RBC diluting fluid
isotonic
high specific gravity
easy to prepare
cheap
good preservative
has a buffer action
not initiate growth of molds
The best RBC diluting fluid wherein the cell shape is not altered
Dacie’s/Formol Citrate
This RBC diluting fluid is disadvantageous as it allows growth of molds
Hayem’s
This RBC diluting fluid is disadvantageous as it produces clumping of cells
Hayem’s
T/F: Both Dacie’s and Hayem’s can be stored for long periods of time
T
T/F: Hayem’s has a corrosive effect
F
This RBC diluting fluid prevents rouleaux formation
Gower’s
This RBC diluting fluid precipitates protein in hemoglobinemia and hyperglobulinemia
Gower’s
This RBC diluting fluid is best for beginners
Toisson’s
This RBC diluting fluid is a stable preservative
NSS
RBC count is multiplied by ___ to calculate the total RBC
10 000
Toisson’s RBC diluting fluid supports the growth of fungi. What is the best thing to do to prevent this?
Filter the diluting fluid
This RBC diluting fluid has a high specific gravity and can stain WBC
Toisson’s
The RBC count in the 5 squares should not have a difference of ___
> 20
This component of Toisson’s diluting fluid aid in WBC and RBC differentiation
Methyl violet
This RBC diluting fluid is used in emergency cases and excessive rouleaux formation
NSS
This RBC diluting fluid is used for autoagglutinated cells
NSS
Reference range of RBC in males
4.5-6.0 M/cumm
Reference range of RBC in female
4.0-5.5 M/cumm
Reference range of RBC in late pregnancy
3.0-5.0 M/cumm
Reference range of RBC at birth
7.0 M/cumm
To calculate for the SI unit of RBC, the conventional unit is multiplied by ____
0.000001
Overcharging in RBC count can produce a falsely ___ result
decreased
How do we troubleshoot when RBC is markedly increased?
decrease the volume of aspirated blood
How do we troubleshoot when RBC is markedly decreased?
increase the volume of aspirated blood
use standard blood volume but increase the number of tertiary squares to count
Formula for RBC count
Total no. of cell counted/ (1/5 x 1/10 x 1/200)
Total no. cell counted / (number of squares x depth x dilution)
Men have ___ RBC counts that women
higher
Adults have ___ RBC counts than newborns
lower
Dehydration and strenuous activities may result to ___ RBC count
increased
It is expected to have a/n ___ RBC count in individuals living at higher altitudes
increased
Pathologic variation in RBC count
Polycythemia
Anemia/Oligocythemia
Hemorrhage
Drugs
Secondary to other disorders/diseases
A convenient RBC counting method wherein the dilution fluid is already pre-measured
Unopette system
These significantly replaced the manual method of RBC counting
Automated analyzers
Principle of automated analyzers for RBC counting
Electronic impedance / Flow cytometry
In the photoelectric method of RBC counting, the ___ is proportional to the number of RBC present
solution turbidity
Automated method of RBC counting:
analyzes and reports CBC, RBC indices, RDW, platelet count, MVP, PDW, PLCR, and nucleated cells according to size
Sysmex E-5000
Automated method of RBC counting:
performs CBC, platelet count, 5-part WBC differential count including the percentages and absolute values of neutrophils, lymphocytes, monocytes, eosinophils, and basophils and calculates RBC indices, RDW, MVP, PDW, PLCR
Sysmex NE-8000
Automated method of RBC counting:
A combination of different hematology instruments capable of performing CBC, RBC indices, RDW, platelet count, 5-part differential, reticulocyte count, preparation of Wedge blood smear, and identification of bar coded blood specimen
Sysmex total hematology system (HS)
Automated method of RBC counting:
the first model of Coulter Counter Analyzers; performs CBC, MCV, MCH, MCHC, platelet count
Coulter Counter Model S
Automated method of RBC counting:
performs all tests analyzed in the Coulter Counter S plus RDW
Coulter Counter Model S Plus
Automated method of RBC counting:
this analyzer is capable of determining Histogram differential aside from the tests performed by the previous models
Coulter Counter Model S Plus IV
Automated method of RBC counting:
classification of WBC using this analyzer is based on the cell’s VCS (volume, conductivity, and scatter) properties
Counter TM STKS and Coulter MAXM
Automated method of RBC counting:
can process 90 samples per hour
Coulter Counter Model S Plus II
Automated method of RBC counting:
can process 115 samples per hour
Coulter Counter Model S Plus III
Automated method of RBC counting:
the amount of blood sample used by this analyzer is reduced to 100uL instead of 1mL of blood required by the other previous models.
Coulter Counter Model S Plus IV
Automated method of RBC counting:
this model performs tests and reports the same as the Coulter Counter Model S Plus IV; the major alteration is the addition of an automated cap piercer used to aspirate the speciment
Coulter Counter S Plus V
Automated method of RBC counting:
the innovation on the model is the addition of an autosampler which is equipped with 2 rotating sample trays that can hold 32 tubes each
Coulter Counter S Plus VI
Automated method of RBC counting:
the said analyzer is capable of accepting and mixing 144 specimens at one time in preparation for specimen identification, aspiration, and analysis
Coulter Counter S Plus STKR
