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[3Y1S] MT6318 > 02 LAB: RED BLOOD CELL COUNT > Flashcards

02 LAB: RED BLOOD CELL COUNT Flashcards

1
Q

RBC Count is the number of RBCs in ___ of blood

A

1 L

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2
Q

In traditional units, RBC count is measured by ___

A

number of cells per cubic millimeter

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3
Q

Preferred tube for RBC count

A

EDTA

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4
Q

Characteristics of RBC diluting fluid

A

isotonic
high specific gravity
easy to prepare
cheap
good preservative
has a buffer action
not initiate growth of molds

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5
Q

The best RBC diluting fluid wherein the cell shape is not altered

A

Dacie’s/Formol Citrate

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6
Q

This RBC diluting fluid is disadvantageous as it allows growth of molds

A

Hayem’s

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7
Q

This RBC diluting fluid is disadvantageous as it produces clumping of cells

A

Hayem’s

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8
Q

T/F: Both Dacie’s and Hayem’s can be stored for long periods of time

A

T

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9
Q

T/F: Hayem’s has a corrosive effect

A

F

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10
Q

This RBC diluting fluid prevents rouleaux formation

A

Gower’s

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11
Q

This RBC diluting fluid precipitates protein in hemoglobinemia and hyperglobulinemia

A

Gower’s

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12
Q

This RBC diluting fluid is best for beginners

A

Toisson’s

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12
Q

This RBC diluting fluid is a stable preservative

A

NSS

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12
Q

RBC count is multiplied by ___ to calculate the total RBC

A

10 000

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12
Q

Toisson’s RBC diluting fluid supports the growth of fungi. What is the best thing to do to prevent this?

A

Filter the diluting fluid

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12
Q

This RBC diluting fluid has a high specific gravity and can stain WBC

A

Toisson’s

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12
Q

The RBC count in the 5 squares should not have a difference of ___

A

> 20

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12
Q

This component of Toisson’s diluting fluid aid in WBC and RBC differentiation

A

Methyl violet

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13
Q

This RBC diluting fluid is used in emergency cases and excessive rouleaux formation

A

NSS

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13
Q

This RBC diluting fluid is used for autoagglutinated cells

A

NSS

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13
Q

Reference range of RBC in males

A

4.5-6.0 M/cumm

14
Q

Reference range of RBC in female

A

4.0-5.5 M/cumm

14
Q

Reference range of RBC in late pregnancy

A

3.0-5.0 M/cumm

15
Q

Reference range of RBC at birth

A

7.0 M/cumm

16
Q

To calculate for the SI unit of RBC, the conventional unit is multiplied by ____

A

0.000001

17
Q

Overcharging in RBC count can produce a falsely ___ result

A

decreased

18
Q

How do we troubleshoot when RBC is markedly increased?

A

decrease the volume of aspirated blood

19
Q

How do we troubleshoot when RBC is markedly decreased?

A

increase the volume of aspirated blood

use standard blood volume but increase the number of tertiary squares to count

20
Q

Formula for RBC count

A

Total no. of cell counted/ (1/5 x 1/10 x 1/20)

Total no. cell counted / (number of squares x depth x dilution)

21
Q

Men have ___ RBC counts that women

A

higher

22
Q

Adults have ___ RBC counts than newborns

A

lower

23
Q

Dehydration and strenuous activities may result to ___ RBC count

A

increased

24
Q

It is expected to have a/n ___ RBC count in individuals living at higher altitudes

A

increased

25
Q

Pathologic variation in RBC count

A

Polycythemia
Anemia/Oligocythemia
Hemorrhage
Drugs
Secondary to other disorders/diseases

26
Q

A convenient RBC counting method wherein the dilution fluid is already pre-measured

A

Unopette system

27
Q

These significantly replaced the manual method of RBC counting

A

Automated analyzers

27
Q

Principle of automated analyzers for RBC counting

A

Electronic impedance / Flow cytometry

27
Q

In the photoelectric method of RBC counting, the ___ is proportional to the number of RBC present

A

solution turbidity

28
Q

Automated method of RBC counting:

analyzes and reports CBC, RBC indices, RDW, platelet count, MVP, PDW, PLCR, and nucleated cells according to size

A

Sysmex E-5000

29
Q

Automated method of RBC counting:

performs CBC, platelet count, 5-part WBC differential count including the percentages and absolute values of neutrophils, lymphocytes, monocytes, eosinophils, and basophils and calculates RBC indices, RDW, MVP, PDW, PLCR

A

Sysmex NE-8000

30
Q

Automated method of RBC counting:

A combination of different hematology instruments capable of performing CBC, RBC indices, RDW, platelet count, 5-part differential, reticulocyte count, preparation of Wedge blood smear, and identification of bar coded blood specimen

A

Sysmex total hematology system (HS)

31
Q

Automated method of RBC counting:

the first model of Coulter Counter Analyzers; performs CBC, MCV, MCH, MCHC, platelet count

A

Coulter Counter Model S

32
Q

Automated method of RBC counting:

performs all tests analyzed in the Coulter Counter S plus RDW

A

Coulter Counter Model S Plus

33
Q

Automated method of RBC counting:

this analyzer is capable of determining Histogram differential aside from the tests performed by the previous models

A

Coulter Counter Model S Plus IV

34
Q

Automated method of RBC counting:

classification of WBC using this analyzer is based on the cell’s VCS (volume, conductivity, and scatter) properties

A

Counter TM STKS and Coulter MAXM

35
Q

Automated method of RBC counting:

can process 90 samples per hour

A

Coulter Counter Model S Plus II

36
Q

Automated method of RBC counting:

can process 115 samples per hour

A

Coulter Counter Model S Plus III

37
Q

Automated method of RBC counting:

the amount of blood sample used by this analyzer is reduced to 100uL instead of 1mL of blood required by the other previous models.

A

Coulter Counter Model S Plus IV

38
Q

Automated method of RBC counting:

this model performs tests and reports the same as the Coulter Counter Model S Plus IV; the major alteration is the addition of an automated cap piercer used to aspirate the speciment

A

Coulter Counter S Plus V

39
Q

Automated method of RBC counting:

the innovation on the model is the addition of an autosampler which is equipped with 2 rotating sample trays that can hold 32 tubes each

A

Coulter Counter S Plus VI

40
Q

Automated method of RBC counting:

the said analyzer is capable of accepting and mixing 144 specimens at one time in preparation for specimen identification, aspiration, and analysis

A

Coulter Counter S Plus STKR

[3Y1S] MT6318 (16 decks)

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