01 LAB: Blood Smear Preparation

Question 1

Complete blood count is also called ___

Answer 1

Hemogram

Question 2

The peripheral blood film is preferably prepared within ___

Answer 2

2-3 hours

Question 3

Simplest and most common method of creating blood films

Answer 3

Two-slide/ Wedge method

Question 4

Wedge technique:

Size of blood: ___
Angle of spreader slide: ___

Answer 4

2-3 mm
30-45 degrees

Question 5

An older technique that is now used rarely for PBS, but is sometimes still used for making bone marrow aspirate smears

Answer 5

Two-cover slip/ Elrich’s two-coverglass method

Question 6

This smearing technique produces an excellent leukocyte distribution, which lends itself to more accurate determination of differential counts

Answer 6

Two-cover slip/ Elrich’s two-coverglass method

Question 7

This smearing technique produces uniform blood film, monolayer of cells distributed accurately

Answer 7

Spinner method

Question 8

This automated smear preparation allows the production of blood smear for every 30 seconds

Answer 8

Sysmex SP-1000i

Question 9

This automated smear preparation allows the creation of four slides from a 90 microliters of sample and 12 slides from a single sample presentation

Answer 9

DxH Slidemaker Stainer II Cellular Analysis System

Question 10

The specific anticoagulant used to observe cell morphology

Answer 10

Ethylenediaminetetraacetic acid (EDTA)

Question 11

Stain used for the preparation of blood smear

Answer 11

Romanowsky stain

Question 12

Eosin and methylene blue stains are ___ stains

Answer 12

Polychrome

Question 13

Anticoagulant NOT used for peripheral blood smears:

Causes blue background

Answer 13

Heparin

Question 14

Anticoagulant NOT used for peripheral blood smears:

Distort cellular morphology

Answer 14

Oxalate and Fluoride

Question 15

Give the reagent and how much time needed:

Used to fix the blood to the slide

Answer 15

Methanol
30 seconds

Question 16

Give the reagent and how much time needed:

Stains basic components such as hemoglobin or eosinophilic granules

Answer 16

Eosin stain
6 seconds

Question 17

Give the reagent and how much time needed:

Stains acidic components such as RNA

Answer 17

Methylene blue
4 seconds

Question 18

Component of buffer solution needed for PBS

Answer 18

0.05 M sodium phosphate or aged distilled water

Question 19

pH level of the buffer solution needed for PBS

Answer 19

6.4

Question 20

Give the stain color in PBS:

RBC

Answer 20

pink to salmon

Question 21

Give the stain color in PBS:

Nuclei

Answer 21

Dark blue to purple

Question 22

Give the stain color in PBS:

Cytoplasmic granules of neutrophils

Answer 22

Lavender to lilac

Question 23

Give the stain color in PBS:

Cytoplasmic granules of basophils

Answer 23

Dark blue to black

Question 24

Give the stain color in PBS:

Cytoplasmic granules of eosinophils

Answer 24

Red to orange

Question 25

Identify the magnification to use:

Validates the overall quality of the film including abnormal distribution of RBCs

Answer 25

10x

Question 26

Identify the magnification to use:

Rapid detection of large abnormal cells

Answer 26

10x

Question 27

Identify the magnification to use:

Quality control tool for validating WBC

Answer 27

40x or 50x

Question 28

Identify the magnification to use:

Used for WBC differential count

Answer 28

100x