Week Seven (Replication)

Question 1

Pyrimidines

Answer 1

Cytosine, thymine, uracil

Question 2

Purines

Answer 2

Adenine, guanine

Question 3

Growing DNA

Answer 3

Triphosphate created by adding a pyrophosphate to an existing monophosphate

Question 4

Importance of DNA being anti-parallel

Answer 4

Correct orientation for primers and new strands

Question 5

Role of enzymes in replication

Answer 5

Recognize origin of replication and other functions in replication

Question 6

Helicase

Answer 6

Unwinds parental double helix at replication forks

Question 7

Single stranded binding proteins

Answer 7

“Holds together”
Binds to stabilize single stranded DNA until needed as a template

Question 8

Topoisomerase

Answer 8

“Unwinds”
Relieves tension, breaking and resealing phosphodiester bonds in DNA

Question 9

Primase

Answer 9

Synthesizes RNA primer for leading and okazoki fragments

Question 10

DNA polymerase 3

Answer 10

“Initial”
Synthesizes new DNA adding to RNA primer

Question 11

DNA polymerase 1

Answer 11

“Clean up crew”
Going in and replacing RNA sections (primers) with DNA

Question 12

DNA ligase

Answer 12

Joins fragments

Question 13

Telomerase

Answer 13

Extends parental strand (bound with RNA template)

Question 14

Okazaki Fragments

Answer 14

Short synthesized fragments in lagging strands