Week 3 - Plasma Membrane Transport & Mitosis Flashcards

1
Q

What is plasma membrane in terms of transport?

A
  • Both a barrier and gateway between cytoplasm and extracellular fluid
  • Some molecules pass easily through, others do not (selectively permeable)
  • Atoms and molecules use Passive or Active processes
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2
Q

What is passive transport?

A
  • No ATP required
  • Molecules moves DOWN their concentration gradient (from high to low)
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3
Q

What is simple diffusion?

A

Non-polar, fat-soluble (hydrophobic) molecules diffuse through phospholipid bilayer
(e.g., oxygen (O2) into a cell)

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4
Q

What is facilitated diffusion?

A
  • Polar (electrolytes), fat-insoluble / water-soluble (hydrophilic) molecules
  • Carrier or channel protein (e.g., glucose)
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5
Q

What is osmosis?

A
  • Simple diffusion of H2O across a selectively permeable membrane
  • Between the phospholipids or Aquaporins (channels)
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6
Q

What is osmolarity?

A
  • Concentration of solute particles (stuff) in solution
  • When solutions of different osmolarity are separated by a plasma membrane, osmosis occurs
  • Water moves DOWN its concentration gradient
  • Until water concentration same on both sides (equilibrium)
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7
Q

What happens when osmosis occurs?

A
  • Water enters or leaves a cell
  • Changing cell volume disrupts cell function
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8
Q

What is tonicity?

A

The ability of a solution to cause a cell to shrink or swell

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9
Q

What is hypotonic?

A

Hypotonic solution has a lesser solute concentration (thus more water) than cell.

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10
Q

What is isotonic?

A

Isotonic solution has same solute concentration (thus water concentration) as that of cell.

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11
Q

What is hypertonic?

A

Hypertonic solution has a greater solute concentration (thus less water) than cell.

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12
Q

What is active transport processes?

A
  • Molecules moves UP their concentration gradient (from low to high)
  • ATP (energy) required
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13
Q

What is vesicular transport?

A
  • Requires ATP
  • Large molecules in vesicles (Sacs)
  • Vesicle phospholipids merge with phospholipids of plasma membrane or other
    membranous structures
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14
Q

What is exocytosis?

A

Secrete molecules out of cell

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15
Q

What is endocytosis?

A

Bring molecules into cell

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16
Q

What is the cell life cycle?

A
  • Many cells have a limited lifespan and need to be replaced e.g., gut lining, wound healing
  • Life cycle length variable between cells
17
Q

What is interphase?

A
  • Housekeeping e.g., protein synthesis
  • DNA replicated to make enough chromosomes for two new cells
18
Q

What is mitosis?

A

One pre-existing cell divides into two new identical cell

19
Q

What is DNA replication?

A
  • DNA untwisted to expose the two nucleotide strands (polymers)
  • Each strand used as a template by DNA polymerase to build a new complementary
    strand
  • Forty-six chromosomes replicated in 6-8 h by thousands of DNA polymerases
20
Q

What happens at the end of DNA replication?

A
  • Each chromosome now composed of two identical chromatids held together at
    centromere
  • 46 chromosomes = 92 chromatids
  • Ready for mitosis (mechanical process)
21
Q

State the steps of the mitosis process?

A
  1. Prophase
  2. Metaphase
  3. Anaphase
  4. Telophase
    (IPMAT)
22
Q

What is prophase?

A
  • Chromosomes compacted
  • Nuclear envelope disintegrates
  • Centrioles sprout microtubules (spindles) which push centrioles to each pole of cell
  • Some microtubules attach the centromeres
23
Q

What is metaphase (middle)?

A

Forty-six chromosomes lined up at cell equator by microtubules (Metaphase plate)

24
Q

What is anaphase (apart)?

A
  • Centromeres split separating the sister chromatids
  • Chromosomes (single chromatids) pulled toward poles
25
Q

What is telophase (end)?

A
  • Nuclear membrane reformed
  • Cytokinesis completes cell division, where cytoplasm pinched into two new daughter cells, genetically identical