Week 3 Molecular biology techniques 2

Question 1

Vector

Answer 1

A DNA molecule that carries a specific segment into a certain cell, i

Question 2

The 3 sections of vectors

Answer 2

Cloning site - DNA is attached
Antibiotic resistant gene - Allows selection of DNA which contains recombinant DNA
Origin of replication - Allows replication

Question 3

The 4 steps of DNA cloning

Answer 3

1. Restriction enzymes cut DNA, the same enzymes cut the vector, both have the same ends so they can be ligated together
2. Transformation - Uses electroporation to create holes in the membrane through which DNA enters
3. Select bacteria with recombinant DNA - If cells replicate, they have a vector
4. Isolating recombinant DNA from the bacteria - Special techniques are used to extract DNA from bacteria

Question 4

How do we view RNA?

Answer 4

Unstable, use verse transcriptase to convert into cDNA, can be analysed using PCR