Week 2 read 5.1-.5.14 Y

Question 1

Name 3 main methods to monitor growth + adv/dis

Answer 1

Direct measurement - Count with chambers, quick but don’t know if cells are dead or alive

2. Viable count Spread on petri dish to incubate, accurate but time consuming
3. Dry weight, Precise but time consuming

Question 2

Main factors in growth

Answer 2

Nutrients temp moisutre stress ph pressure atmosphere

Question 3

Necessary enzymes need for growth

Answer 3

SOD- super oxide dismutase, catalase

Question 4

Chemoautotrophs

Answer 4

makes energy

Question 5

Chemoheterotrophs

Answer 5

Finds energy

Question 6

Binary fission

Answer 6

Cell replicates: Chromosome replicated, cell elongates, wall in middle,splits

Question 7

Difference in binary fission and mitosis

Answer 7

BF in procaryotes/ bactieria Mitosis in eukaryotes

BF less complicated

BF in cytoplasm Mitosis in Nucleus

Question 8

Chemostat

Answer 8

Culture device to grow bacteria

Question 9

Cardinal temp

Answer 9

Max min and opt temperature for organism

Question 10

Extremophiles

Answer 10

high heat

Question 11

Thermophiles

Answer 11

high heat but lower

Question 12

Hyperthermophile

Answer 12

X treme

Question 13

Mesophiles

Answer 13

mid

Question 14

Pyscho

Answer 14

low

Question 15

Organisms with an optimum growth of 10 < called

Answer 15

Pyschorphiles

Question 16

A culture in a closed vessel to which no additional nutrients are added and from which no waste product is removed is called

Answer 16

A batch culture

Question 17

An open system in which the growth rate is controlled be adding nutrient (present in limiting quantities) at the same rate that medium containing microorganisms is removed is called

Answer 17

a chemostat

Question 18

Photoorgano(heter)trophs sources of energy, electron/hydrogen and carbon are

Answer 18

Light organic H + 02

Question 18

In a one-step growth experiment, the time during which infective virions seemingly disappear form the cell culture medium is called the

Answer 18

eclipse period

Question 19

Some bacteria are flagellated with flagella inserted at many points evenly distributed around the cell surface. What is this type of flagellation called?

Answer 19

Peritrous

Question 20

T or F Most bacteria can grow well below pH 5.0

Answer 20

F

Question 21

Which of the following can be used to isolate pure cultures from a mixed bacterial consortium.

Spread plates (b) Streak plates (c) Pour plates
(d) All of the above (e) None

Answer 21

All

Question 22

Binary fission steps 5

Answer 22

1. DNA is copied producing 2 identical molecules in membrane
2. Chromosomes are segregated on opposite sides
3. Cell elongates
4. Septum / wall splits the membrane in half
5. Cell divides into 2 daughter cells

Question 23

Name the 4 phases

Answer 23

Lag - Little to no microbial growth, microbes getting use to the environment

Log - Highest rate,

Stationary - Slows due to less products and more waste, Death=Creation

Death - Waste products cause death

Question 24

Osmotic pressure

Answer 24

Pressure of water across a semi permeable membrane

Question 25

Batch culture

Answer 25

Closed system where micro organisms are in a fixed volume without adding or removing products

Question 25

Continuous culture

Answer 25

Open system where nutrients and products are constantly being removed and added

continuous log phase

Question 25

Filtration

Answer 25

Laboratory technique to separate molecules

Passes through a filter, molecules that get too big can’t fit through

Used in water sterilisation