Week 2 read 5.1-.5.14 Y

Question 1

What is microbial growth

Answer 1

Increase in population

Question 2

Types to grow culture and what they are

Answer 2

1.Batch culture - Isolate in dish with 4 methods: Lag adapts to environment, Log cells grow, Stationary both rates the same Death, more cells die

2. Continuous growth -Open system - nutrients supplied and waste removed
3. Diauxic growth - uses two energy sources, uses up one THEN the other

Question 3

Name 3 main methods to monitor growth + adv/dis

Answer 3

Direct measurement - Count with chambers, quick but don’t know if cells are dead or alive

2. Viable count Spread on petri dish to incubate, accurate but time consuming
3. Dry weight, Precise but time consuming
4. Turbudity Change in light uses spectrophotometer, quick bet measures ALL cells

Question 4

Main factors in growth

Answer 4

Nutrients temp moisutre stress ph pressure atmosphere

Question 5

Necessary enzymes

Answer 5

SOD- super oxide dismutase, catalase

Question 6

Chemoautotrophs

Answer 6

makes energy

Question 7

Chemoheterotrophs

Answer 7

Finds energy

Question 8

Binary fission

Answer 8

Cell replicates: Chromosome replicated, cell elongates, wall in middle,splits

Question 9

Difference in binary fission and mitosis

Answer 9

BF in procaryotes/ bactieria Mitosis in eukaryotes

BF less complicated

BF in cytoplasm Mitosis in Nucleus

Question 10

Chemostat

Answer 10

Culture device to grow bacteria

Question 11

Cardinal temp

Answer 11

Max min and opt temperature for organism

Question 12

Extremophiles

Answer 12

high heat

Question 13

Thermophiles

Answer 13

high heat but lower

Question 14

Hyperthermophile

Answer 14

X treme

Question 15

Mesophiles

Answer 15

mid

Question 16

Pyscho

Answer 16

low

Question 17

Organisms with an optimum growth of 10 < called

Answer 17

Pyschorphiles

Question 18

A culture in a closed vessel to which no additional nutrients are added and from which no waste product is removed is called

Answer 18

A batch culture

Question 19

An open system in which the growth rate is controlled be adding nutrient (present in limiting quantities) at the same rate that medium containing microorganisms is removed is called

Answer 19

a chemostat

Question 20

Photoorgano(heter)trophs sources of energy, electron/hydrogen and carbon are

Answer 20

Light organic H + 02

Question 20

In a one-step growth experiment, the time during which infective virions seemingly disappear form the cell culture medium is called the

Answer 20

eclipse period

Question 21

Some bacteria are flagellated with flagella inserted at many points evenly distributed around the cell surface. What is this type of flagellation called?

Answer 21

Peritrous

Question 22

T or F Most bacteria can grow well below pH 5.0

Answer 22

F

Question 23

Which of the following can be used to isolate pure cultures from a mixed bacterial consortium.

Spread plates (b) Streak plates (c) Pour plates
(d) All of the above (e) None

Answer 23

All