Week 2

Question 1

purpose of week 2

Bacterial Transformation?

Answer 1

uptake of exogenous naked dna of cells.
this new dna will become HERITABLE PART of cells genetic material

Question 2

DNA?

Answer 2

• deoxyribonucleic acid
• contains genetic material/instructions

Question 3

RNA

Answer 3

messenger that carries out DNA instructions for controlling the synthesis of protiens

Question 4

proteins

Answer 4

Monomer= amino acids

Question 5

Makeup of amino Acid

Answer 5

1. acidic group (carboxyl gorup -COOH)
2. Amino group (H2N
3. R group (varies and determines its identity

Question 6

Are Bacteria Prokaryotes or Eukaryotes

Answer 6

Prokaryotes

Question 7

Bacteria composition

Answer 7

• no nucleus
• dna is organized in one double stranded circulor molecul known as NUCLEOID

Question 8

bacteria host organism we will study in lab

Answer 8

E choli

Question 9

E choli

Answer 9

Most studied bacteria,
grows extremely fast
harmless under normal conditions

Question 10

how did we use bacteria in lab

Answer 10

insert new dna in bacteria so it produces whaterver we want to mass produce
ie.. protiens like insulin
modified foods broccolli
bio remediation

Question 11

Lab experiment

Answer 11

1) labeled one postiive and negative tube
2) added cacls to neutralize negative charge so plasmid can absorb
3) take ecoli from starter plate use inoculate loop of agar plate
4) then add pblu dna and incubate in ice for 15 min\wait
5) then heat shock for 90 second to get bacteria in plasmid
6) place back in ice for 1-2 mn
7)add liquid liria (food)
8)incubate in room temp for 5-15 min
9)plate contents from tube put onto - and + agar plates and incubate at 37 degrees for 24 hours

Question 12

How will we know bacteria will uptake

Answer 12

growth/colonies

Question 13

Predictive results from lab

Answer 13

Positive control (with pBlu plasmid):

Ampicillin + X-Gal LB: Bacteria will survive and turn blue.
Ampicillin LB: Bacteria will survive but will not turn blue.
Negative control (no plasmid):

Ampicillin + X-Gal LB: Bacteria will not survive and will not turn blue.
Ampicillin LB: Bacteria will not survive and will not turn blue.

Question 14

correct test results from lab

Answer 14

Correct Test Results:
Positive control (with plasmid):

Ampicillin + X-Gal LB → + (blue)
Ampicillin LB → + (not blue)
Negative control (no plasmid):

Ampicillin + X-Gal LB → - (no growth)
Ampicillin LB → - (no growth)

Question 15

what conclusion could u draw if u do not get the predictive results

Answer 15

• cross contamination
• bacteria did not take up plasmid due to innacurate timing of ice before and after heat shock
• broth was not added

Question 16

what are testing in this experiment in terms of bacteria

Answer 16

if bacteria is ampicillin resistant or not
if grown has amp resistance if not does not have gene for it