Week 10 - Stem Cells

Question 1

cell from mesoderm

Answer 1

muscle

Question 2

muscle comes from ______ derm

Answer 2

meso

Question 3

pancreas and liver comes from _______ derm

Answer 3

endo

Question 4

brain and skin comes from ______ derm

Answer 4

ecto

Question 5

blue marker cell

Answer 5

laxZ / betagalactosidase

Question 6

in the mouse experience, a mammary system was made from one ______

Answer 6

stem cell

Question 7

in the mouse model, did the stem cell make other stem cells?

Answer 7

yes

Question 8

stem cell definition

Answer 8

not differentiated
can divide without limits
divide asymmetricall
self renewal

Question 9

in general, do differentiated cells proliferate?

Answer 9

no

Question 10

a cell that can give rise to different tissues in a certain lineage (mammary, skin, heart, brain) is considered _______

Answer 10

multipotent

Question 11

cell that can give rise to any type of cell

Answer 11

pluripotent

Question 12

bone marrow contains _____ ______ for the entire hematopoietic system

Answer 12

stem cells

Question 13

neural stem cells in the brain generate ____, ____ , ______

Answer 13

neurons, astrocytes, oligodentrocytes

Question 14

if a multipotent hematopoeitic stem cell is placed in the bone marrow, what will happen?

Answer 14

it will give rise to the entire immune system

Question 15

there is a _____ but _____ proliferation of neural stem and progenitor cells

Answer 15

continuous and slow

Question 16

area where the stem cells receive local signals

Answer 16

the niche

Question 17

stem cells stay close to the _____

Answer 17

niche

Question 18

what will an injury cause stem cells to do?

Answer 18

differentiate less, and create more daughter stem cells

Question 19

with no niche signals , the stem cells will ________

Answer 19

differentiate

Question 20

stem cells are immortal , but only under control of _____ ______ ______

Answer 20

external limiting signals

Question 21

FACS and use

Answer 21

fluorescence activated cell sorting, isolation of cells followed by transplantation in lab

Question 22

stem cells in the intestine reside in the ______

Answer 22

crypt

Question 23

IPS cells

Answer 23

induced pluripotent stem cells (activated 4 genes)

Question 24

ICM

Answer 24

inner cell mass. Undifferentiated cells that will give rise to the embryo

Question 25

ES

Answer 25

embryonic stem cells - can be cultured in a dish

Question 26

a blastocyst can be taken out of a pregnant animal, and then…?

Answer 26

implanted in a foster mother

Question 27

cells can be transferred from one blastocyst to another to derive ________ animals

Answer 27

chimeric

Question 28

if you move a blastocyst to another animal, every tissue is ______

Answer 28

chimeric

Question 29

an oocyte can ______ a nucleaus from a somatic cell

Answer 29

reprogram

Question 30

how many genes were used to created pluripotent stem cells from a somatic cell?

Answer 30

4 genes

Question 31

3 ways to create stem cells

Answer 31

1. in vitro fertilization
2. nuclear transfer - put nucleus for somatic cell into oocyte
3. IPS cells , express 4 genes