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HumBio 3A - 2nd Half > Week 10 - Stem Cells > Flashcards

Week 10 - Stem Cells Flashcards

1
Q

cell from mesoderm

A

muscle

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2
Q

muscle comes from ______ derm

A

meso

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3
Q

pancreas and liver comes from _______ derm

A

endo

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4
Q

brain and skin comes from ______ derm

A

ecto

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5
Q

blue marker cell

A

laxZ / betagalactosidase

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6
Q

in the mouse experience, a mammary system was made from one ______

A

stem cell

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7
Q

in the mouse model, did the stem cell make other stem cells?

A

yes

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8
Q

stem cell definition

A

not differentiated
can divide without limits
divide asymmetricall
self renewal

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9
Q

in general, do differentiated cells proliferate?

A

no

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10
Q

a cell that can give rise to different tissues in a certain lineage (mammary, skin, heart, brain) is considered _______

A

multipotent

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11
Q

cell that can give rise to any type of cell

A

pluripotent

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12
Q

bone marrow contains _____ ______ for the entire hematopoietic system

A

stem cells

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13
Q

neural stem cells in the brain generate ____, ____ , ______

A

neurons, astrocytes, oligodentrocytes

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14
Q

if a multipotent hematopoeitic stem cell is placed in the bone marrow, what will happen?

A

it will give rise to the entire immune system

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15
Q

there is a _____ but _____ proliferation of neural stem and progenitor cells

A

continuous and slow

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16
Q

area where the stem cells receive local signals

A

the niche

17
Q

stem cells stay close to the _____

A

niche

18
Q

what will an injury cause stem cells to do?

A

differentiate less, and create more daughter stem cells

19
Q

with no niche signals , the stem cells will ________

A

differentiate

20
Q

stem cells are immortal , but only under control of _____ ______ ______

A

external limiting signals

21
Q

FACS and use

A

fluorescence activated cell sorting, isolation of cells followed by transplantation in lab

22
Q

stem cells in the intestine reside in the ______

A

crypt

23
Q

IPS cells

A

induced pluripotent stem cells (activated 4 genes)

24
Q

ICM

A

inner cell mass. Undifferentiated cells that will give rise to the embryo

25
Q

ES

A

embryonic stem cells - can be cultured in a dish

26
Q

a blastocyst can be taken out of a pregnant animal, and then…?

A

implanted in a foster mother

27
Q

cells can be transferred from one blastocyst to another to derive ________ animals

A

chimeric

28
Q

if you move a blastocyst to another animal, every tissue is ______

A

chimeric

29
Q

an oocyte can ______ a nucleaus from a somatic cell

A

reprogram

30
Q

how many genes were used to created pluripotent stem cells from a somatic cell?

A

4 genes

31
Q

3 ways to create stem cells

A
  1. in vitro fertilization
  2. nuclear transfer - put nucleus for somatic cell into oocyte
  3. IPS cells , express 4 genes

HumBio 3A - 2nd Half (17 decks)

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