Week 10 - Stem Cells Flashcards
cell from mesoderm
muscle
muscle comes from ______ derm
meso
pancreas and liver comes from _______ derm
endo
brain and skin comes from ______ derm
ecto
blue marker cell
laxZ / betagalactosidase
in the mouse experience, a mammary system was made from one ______
stem cell
in the mouse model, did the stem cell make other stem cells?
yes
stem cell definition
not differentiated
can divide without limits
divide asymmetricall
self renewal
in general, do differentiated cells proliferate?
no
a cell that can give rise to different tissues in a certain lineage (mammary, skin, heart, brain) is considered _______
multipotent
cell that can give rise to any type of cell
pluripotent
bone marrow contains _____ ______ for the entire hematopoietic system
stem cells
neural stem cells in the brain generate ____, ____ , ______
neurons, astrocytes, oligodentrocytes
if a multipotent hematopoeitic stem cell is placed in the bone marrow, what will happen?
it will give rise to the entire immune system
there is a _____ but _____ proliferation of neural stem and progenitor cells
continuous and slow
area where the stem cells receive local signals
the niche
stem cells stay close to the _____
niche
what will an injury cause stem cells to do?
differentiate less, and create more daughter stem cells
with no niche signals , the stem cells will ________
differentiate
stem cells are immortal , but only under control of _____ ______ ______
external limiting signals
FACS and use
fluorescence activated cell sorting, isolation of cells followed by transplantation in lab
stem cells in the intestine reside in the ______
crypt
IPS cells
induced pluripotent stem cells (activated 4 genes)
ICM
inner cell mass. Undifferentiated cells that will give rise to the embryo
ES
embryonic stem cells - can be cultured in a dish
a blastocyst can be taken out of a pregnant animal, and then…?
implanted in a foster mother
cells can be transferred from one blastocyst to another to derive ________ animals
chimeric
if you move a blastocyst to another animal, every tissue is ______
chimeric
an oocyte can ______ a nucleaus from a somatic cell
reprogram
how many genes were used to created pluripotent stem cells from a somatic cell?
4 genes
3 ways to create stem cells
- in vitro fertilization
- nuclear transfer - put nucleus for somatic cell into oocyte
- IPS cells , express 4 genes
