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Phlebotomy > waived testing > Flashcards

waived testing Flashcards

1
Q

What are waived testing procedures

A
  • at home
  • using kits that ae availalbe from number of manufactureres
  • includes easy to follow isntruction and most come with b uilt in control
  • includes:
    ● erythrocyte sedimentation rate (ESR)
    ● fecal occult blood testing
    ● microhematocrit
    ● strep screening
    ● urine pregnancy testing
    ● urine chemical screening
    ● point-of-care testing (POCT)
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2
Q

what are the levels of lab testing designated by the CLIA in 1988`

A
  • waived tests, moderate complecity tests and high complixty testing, provider-performed microscopy
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3
Q

what are high complexity tests

A
  • tests that require close attention to details
  • require manual manpulation of high complexd equipment and reagenets (lab test chemicals) and that require interpretation and troubleshoot skills
  • manual dna extraction procedures intricate special staining procuedre and operation of complex analyzer that require detailed setup or ieator interactions
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4
Q

what are moderate complexity tests

A
  • faled btwn waived and high compleicty
  • a few orcoedural step that aer not highly complexy but requre some forma training to perform
  • include running automated instruments (little manual manipulatio of specimen or reagenet with minimal interpretation and tourbleshooting skiills)
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5
Q

what is certificate of accrediation?

A
  • moderate and or high complex testing
  • private not for profit accreditation program
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6
Q

what are certificate of compliance

A

moderate and or high complex testing
lab is in compliance with all applicable CLIA requirements
- labs r surveyed every other year

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7
Q

certificate of registration

A
  • lab that applied for COA COC
  • lab can perform moderate and or high complex testing until inspected for COA or COC
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8
Q

Certificate for Provider-Performed Microscopy Procedures

A
  • lab where physician midlevel practitioner or dentist perform only microscopy procuedres q
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9
Q

certificate of waiver

A
  • lab performs only waived tests
  • must ably to department of health and human services, centers for medicare and medicaid services (CMS)
  • submit to randsom inspection and investigatoon
  • recommended to follow Clinical Laboratory Improvement Advisory Committee (CLIAC) suggestions for lab managements during before and after test
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10
Q

provider-performed microscopy procedures
(PPMPs)

A

● direct wet mounts
● potassium hydroxide (KOH) preparations
● pinworm examinations
● fern tests
● post-coital qualitative
● urine sediment examinations
● nasal smears for granulocytes
● fecal leukocyte examinations
● qualitative semen evaluation

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11
Q

what is lab management and personnel need to adher to ?

A

● Follow all applicable federal, state, and local regulations.
● Perform waived tests only.
● Follow the manufacturer’s instructions in the package insert.
● Do not make modifications to the instructions.
● Allow random inspections by authorized agencies, such as the CMS.
● Establish a laboratory safety plan that follows OSHA guidelines.
● Have a designated area that has adequate space and conditions.
● Have enough personnel in the lab and train them appropriately.
● Have written documentation of each test performed.
- follow standard operating procedures (SOPs)

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12
Q

what is ESR?

A
  • rate at which RBC settle in whole blood
  • measures distance in mL that RBC fall in 1 hr when allowed to settle in a calibrate dtube
  • screens for presence of any inflammation
  • number of plasma proteins such as albumin and globulin increases
  • increase in plasma proteins may = rouleaux formation (RBC sticks together)
  • several cells sticking together settls faster than a single RBC
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13
Q

What are the different methods to perform ESR

A
  • Wintrobe
  • Westergren
  • Modified Westergren
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14
Q

tube and testing for ESR

A
  • requires light blue top tube or their own speciality tube usually with a black stopper or top
  • ESR procedure are done in less than 4 hours but if refrigerated up to 12 hours
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15
Q

what is required for ESR

A

● Specimen transfer pipets
● ESR kit
● ESR vials containing a premeasured amount of diluent (usually 0.9%
sodium chloride)
● Calibrated ESR tubes
● ESR testing rack

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16
Q

how to test ESR

A
  1. Transfer the mixed blood from an appropriate specimen to the diluent vial and fill it
    to the mark on the vial (amounts vary by manufacturer).
  2. Replace the vial cap and gently mix the blood with the diluent by inversion.
  3. Insert a calibrated ESR tube through the vial cap and into the blood–diluent mix-
    ture; adjust the tube until the blood is even with the 0-mm mark.
  4. Place the ESR tube in the testing rack (it should be absolutely level).
  5. Label the ESR tube or vial with the patient’s identification.
  6. Allow the blood to settle for 1 hour.
  7. After 1 hour, read the level to which the red blood cells have fallen and record this
    information as millimeters per hour.
  8. Consult the manufacturer’s instructions for proper interpretation of the results. Nor-
    mal values vary by method and may vary by gender.
17
Q

what could affect ESR results

A
  • colllection
  • testing
  • testing time
  • temp (69-77F is opimtal)
  • tilting (tilting = RBC to settle faster)
  • vibration (settle faster - not same as centrifue_
  • direct sunlight or drafts
  • at room temp longer than 4 hours = lower than act
  • sooner than 1 hr may = lower ESR or if after 1 hour than falsely high ESR
18
Q

what is fecal occult blood testing

A
  • blood in stool that isnt visible
  • fecal occult blood may be sign of infection, inflammatory conditions, trauma, ulcers, hemorrhods, colorectal cancer
  • although high specific tests such as fecal immunohistochemical tests r availabe common waived methods may be used
19
Q

what is the waived method for occult blood

A
  • cardboard holder with paper with guaiac (turn blue when blood is present)
  • several portion of stool is sampled
  • apply thin layer to front of guaiac card then few drops of hydrogen peroxide on back of card
  • if sufficient amount of hemoglobin is present guaic paper (false neg if v small hemoglobin or not enough stool)
  • most of times 3x stool sample
20
Q

false positivefecal occult blood

A
  • fal positive if another type of peroxidase or pseudoperoxidase (enzyme present in
    some foods) is present
  • could occur if pt injest fish, meat with lots of heme (beef and lamb), or food that contain peroxidaise (some fruits and vegetables - bananas, cantaloupe, pears, and
    plum and broccoli, tomatoes
    (including tomato sauce), cauliflower, horseradish, and turnips)
  • avoid thesee footds and aspriirn and vitamin C
21
Q

what is microhematocrit ?

A
  • screening for anemia and is measured as the packed cell volume (PCV) of RBC or percent of RBC in whole blood
  • micro requires small amt of blood
  • uses capillary tubes (narrow with red basnd arund one end, indicating theat tehy are coated with heparin) = blue band means no anticoagulants but could be used for microhematocrit if obtained from an EDTA tube
22
Q

how is microhematocrit testing done?

A
  • directly on dermal puncutre or in microcollection container or evacuated tubes
  • avoid air to enture tuve
  • fill 2 capillaryt tuvbes 3/4 full
  • dont remove tip from blodo source with tuvbe lower tha nblood as air will ender
  • place gloved finger over dry end (not blood collection side) and hold horizontally - remove finger and embed clean end in clay sealent
  • some microhematocrit tubes r self sealing
  • place in larger tube
    microhematocrit centriufge is used to obtain packed RBC
23
Q

how to do strep screening?

A
  • see if group A strep in throught
  • immunoassay test that use antibodies to bacterial antigen to creat e a reaction such as a color change
  • throat swab in testing vial that contains premeasured amt of extraction reagent
  • set on device with antiobides to bacterial antigens and color developers
  • letter t = indication of pt specimen
  • c is contorl
24
Q

What is urine pergnancy testing ?

A
  • tests human chorionic gonadotropin (hCG) which is detecatable in urine at 10 days after pregancy
25
Q

what is urine chemical screenign

A
  • part of urinalysis
  • contains of a a physical
    component (color and clarity), a chemical component (pH, specific gravity, blood, bilirubin, glocuse, ketones, leukocytes, protein, nitries, and urobiligin), and a microscopic component (spinning urine to view sediment under microscope for various cells, crystals, microrganisms, and urine casts - no a waived test )
    -
26
Q

What is a dipstick test?

A
  • plastic strip wit hreagnet pads
  • ## pads have chemical that reactions with a paritcular substnacein urine and hcange color in a precise ways (indiacted presence, amt or concentration of sample)
27
Q

what is Point-of-care testing (POCT)

A
  • near pt testing
  • reduce heatlchare costs while enchaing pt care by making results avialbe quickly
  • collecting specimen and immediately testin on instrument by pt side
  • usually require small amt of blood from dermal puncture
28
Q

examples of POCT blood tests

A

glucose, hemoglobin, sodium, potassium, chloride, bicarbonate, ionized calcium, cholesterol, blood ketones, blood
gases, and coagulation studies, such as prothrombin time (PT)

29
Q

other types of POCT tests?

A

urine
dipstick, urine pregnancy and ovulation tests, fecal occult blood, and screening for infections, such as Group A Streptococcus.

30
Q

what is glucose POCT testing

A
  • screnning for abn glucose levels and monitor glucose levels in DM pt
  • can do at home
31
Q

what is “B-Type Natriuretic Peptide”

A
  • its a cardiac hormone produced by the heart in response to ventricular volume expansion and pressure overload
  • It is the first objective measurement for congestive heart failure (CHF).
  • helps differentiate (COPD) and CHF. - BNP blood concentrations increase with the increasing severity of CHF and have been shown to more accurately reflect final diagnosis than echocardiographic ejection fractions.
32
Q
A

Phlebotomy (17 decks)

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