W1 Tissue Bio Intro Flashcards
what is histology
microanatomy of cells, tissues and organs
correlation between function and structure
what is pathology
the study and diagnosis of diseases
what is physiology
understanding the mechanism of living things
what are the 5 types of tissues
blood
connective
muscle
nervous
epithlia
how is unfixed tissue tackled by the body
degraded by autolysis
what is cut up tissue sampling
remove small pieces of tissue and place into cassettes
what is a fixative
maintain tissues with denature/cross-link proteins
what is the most common fixative
formalin
what is formalin made up of
37% formaldehyde
what is the 3 steps of processing and embedding tissue samples
- dehydration
- clearing
- infiltration with wax
what are tissues further embedding into
paraffin blocks
how is sectioning of tissues done by
microtome cut out 0.004mm sections of tissue
how are sectioned tissues analysis and viewed
mounted onto a glass slide
stained and viewed using light microscope
what is Haematoxylin and Eosin (H and E)
histological staining technique
how is H and E able to stain
H = binds to -ve charged tissue
E = binds to +ve charged tissue
what is basophilia
the binding of basic dye
forms a blue colour
what is basophilia made up of
nuclear chromatin
cytoplasmic RNA
extracellular matrix proteins
what is acidophilia
the binding of acidic dye
forms a pink colour
what is acidophilia made up of
cytoplasmic proteins
intracellular membranes
extracellular protein fibres
what are tissues impregnated into
epoxy resin
what are the 3 histology staining
tinctorial
histochemical
immuno/nucleic
