Visualization Techniques Flashcards
Immunocytochemistry
Labels proteins using antigen cells
In situ hybridization
Labels DNA, RNA, and nucleic acids
How does signal amplification work?
Use secondary antibodies where multiple fluorescent probes can bind to primary antibody to amplify fluorescent signal
How is ICC used to visualize cell activity?
By labeling proteins, ICC allows a person to see what proteins are expressed in a certain cell
What is the difference between fluorescent microscopy and autoradiography?
Fluorescent microscopy used fluorescent probes while autoradiography uses radioactive isotopes
How can fluorophores be introduced into an organism
By linking them to antibodies or proteins
In vivo vs. In vitro
What permits the targeting of genetically selective group of cells?
By creating a fluorescent tag that only binds to a certain protein expressed in targeted cells
How does Brainbow work?
Relies on Cre-Lox recombination where Cre Recombinase drives inversion and excision of DNA between marked sites. Resulting in varying degrees of expression of fluorescent
