Microscopy

Question 1

Which type of microscopy permits high resolution imaging in a single plane?

Answer 1

Confocal Microscopy

Question 2

How does confocal microscopy improve resolution from fluorescent?

Answer 2

Detects light from a single layer of the specimen and restricts the path of light using a pinpoint disc

Question 3

What types of microscopy generate images based on light absorption

Answer 3

Light, fluorescent, and confocal

Question 4

Disadvantages of fluorescent microscopy

Answer 4

Limit on resolution and magnification

Autofluorescence, photo bleaching, photo toxicity

Question 5

Disadvantages of electron microscopy

Answer 5

Only observe dead specimens

Expensive

Limited number of targets

Mostly structural imaging

Question 6

Why is electron microscopy the highest resolution form?

Answer 6

Because it utilizes electrons instead of photons. Electrons have a shorter wavelength resulting in higher resolution

Question 7

Which form of microscopy permits visualization of organelles

Answer 7

Electron

Question 8

How does CLARITY improve resolution?

Answer 8

Removes myelin and lipids