Cell Staining Techniques Flashcards
1
Q
Golgi Stain
A
Whole cell stain using silver particles stains 5-10% of neurons randomly
2
Q
Golgi-Cox Stain
A
Whole cell staining using mercury results in higher resolution than regular golgi
3
Q
DAPI
A
Stains double stranded DNA
Cell permeable
4
Q
Hoescht
A
Stains double stranded DNA
Cell permeable
Used on live cells due to higher permeability
5
Q
PI Stain
A
Stains double stranded DNA
Not cell impermeable
6
Q
Nissle
A
Stains RNA
7
Q
Difference between Golgi and Golgi-Cox again
A
Golgi uses silver to impregnate cells. Golgi-Cox uses mercury and has a higher resolution
8
Q
What is the difference between Hoechst and DAPI stains?
A
Hoechst is used in live cells due to high cell permeability.
DAPI is used on fixed cells
