Virology Flashcards
If you want to detect earlier infections using antibody tests, what antibody needs to be tested for?
(IgM)
Do you look for more or less color to indicate a high antibody load for the following tests:
Direct ELISA
Indirect ELISA
Competitive ELISA
Direct ELISA (more)
Indirect ELISA (more)
Competitive ELISA (less)
The FIV snap test is an example of a lateral flow assay in which antibody/antigen (choose) is detected with a capture antibody/antigen (choose).
(The FIV snap test is an antibody lateral flow assay so it uses FIV antigen to detect antibodies in the patient)
What type of test is the coggins test used for equine infectious anemia?
(Agar gel immunodiffusion test)
Describe how a hemagglutination inhibition test works.
(Patient blood is mixed with the virus, if the patient has no antibodies the virus will lyse the RBCs and no agglutination will occur, if the patient has antibodies then the blood will agglutinate as you expect)
The parvo snap test is an example of a lateral flow assay in which antibody/antigen (choose) is detected with a capture antibody/antigen (choose).
(The parvo snap test is an antigen lateral flow assay so it uses labeled antibodies to detect parvo antigen in the patient)
Which types of ELISA can be used to detect antigen?
(Capture or sandwich)
Fluorescent antibody testing uses labeled antibody/antigen (choose) to detect target antibody/antigen (choose) in tissue samples.
(Uses labeled antibody to detect target antigen in tissue samples → good example is rabies)
Immunohistochemistry testing uses antibody/antigen (choose) to detect target antibody/antigen (choose) in tissue samples.
(Uses antibody to detect target antigen in tissue samples → examples are FIP and Marek’s dz)
What type of sequencing test can be used on impure samples?
(Metagenomic sequencing)
What specifically is histopathology useful for in relation to virus detection?
(Looking for inclusion bodies)
