Serology + Anatomic Pathology

Question 1

What is immunodiagnostics?

Answer 1

(The measurement of antigen-antibody interactions for diagnostics purposes)

Question 2

What is serology?

Answer 2

(Primarily, measuring antibodies in body fluids)

Question 3

Immunodiagnostics can use the detection of antibodies to determine exposure/disease/immunity (choose) in your patient.

Answer 3

(All of the above)

Question 4

(T/F) Presence of antibodies = infection = disease.

Answer 4

(F)

Question 5

This characteristic of a test is the test’s ability to designate an individual with disease/exposure as positive, truly identifying disease.

Answer 5

(Sensitivity → also your false negative rate (if something is 98% sensitive, your false negative rate is 2%))

Question 6

This characteristic of a test is the test’s ability to designate an individual who does not have a disease/exposure as negative, truly identifying lack of disease.

Answer 6

(Specificity → also your false positive rate (if something is 96% specific, your false positive rate is 4%))

Question 7

Which of the fluorescent assays (direct/indirect) available can identify the presence of antigen in tissue?

Answer 7

(Both direct and indirect fluorescent antibody tests)

Question 8

You use a direct/indirect (choose) fluorescent antibody test to measure antibodies in serum.

Answer 8

(Indirect, direct can only be used for detection of antigen in tissues)

Question 9

(T/F) Immunoenzyme assays (ELISA) can measure both antigen and/or antibodies.

Answer 9

(T)

Question 10

Agglutination tests are used to test for the presence of antigen/antibodies (choose) by measuring agglutination using blood or other body fluids (e.g. milk).

Answer 10

(Antibodies)

Question 11

Viral neutralization assays measure antigen/antibodies (choose) and can be quantitative.

Answer 11

(Antibodies, specifically antibodies that neutralize the virus, since these tests are quantitative you can get titer results)

Question 12

(T/F) The color of a positive dot on a SNAP test correlates to the amount of antibodies present in the sample run.

Answer 12

(F, SNAP tests are qualitative only)

Question 13

If you have a huge lesion that you cannot possibly send the entirety of to the lab, what is a key location that you should sample?

Answer 13

(The interface from normal to abnormal tissue)

Question 14

What are two downsides to small samples such as a tru cut biopsy?

Answer 14

(The small sample may not be representative of the entire lesion and small samples are much more susceptible to artifact)

Question 15

When should you sample the GIT in a necropsy?

Answer 15

(At the end optimally)

Question 16

What is the appropriate tissue:formalin ratio?

Answer 16

(1:10)

Question 17

What are the 5 important components of a morphological diagnosis?

Answer 17

(Pathological process, organ affected, chronicity, distribution, and severity)