Virology

Question 1

Property of virus

Answer 1

Obligate intracellular parasite

Either rna or DNA will be there not both

Genome surrounded by capsid
Capsid by the envelope

Smaller in size
Lack the enzymes necessary fr proteins and nucleus acid synthesis

No cellular organisation

No binary fission

Do not grow in media culture

Resistance to antibiotics

They have host specificity

Question 2

Morphological features of viruses

Answer 2

Smallest = parvovirus

Large = pox viruses

Question 3

Structure of viruses

Answer 3

Central core of nuclei acid genome

Genome by proteins coat made of capsomers

Together known as capsid

Question 4

Functions of capsid

Answer 4

Impermeable shell

Introduce viral genome into host by absorbing the host cell membrane

Capsid bilayered lipoprotein

Virus codes glycoproteins subunits on the surface of envelope
Peplomers in the influenz as H and N

Question 5

Symmetry

Answer 5

Icasohedral

Helical some rna viruses

Complex=pox virus

Question 6

Bullet shape virus

Answer 6

Rabies virus

Question 7

Rod shape

Answer 7

Tobacco mosaic viruses

Question 8

Pox shape virus

Answer 8

Brick shaped

Question 9

Adeno

Answer 9

Space vehicle

Question 10

Filamentous shape virus

Answer 10

Marburg and Ebola virus

Question 11

At what temperature viruses are inactiveated an

Answer 11

56*c for 30 min

Question 12

Stable viruses at

Answer 12

40-70*c by lyophilisation

Question 13

What kind of rays inactivate the viruses

Answer 13

Uv rays, X-rays,gamma rays

Question 14

Lipid solvent that destroys viruses

Answer 14

Chloroform,ether,bile salts destroy the enveloped viruses

Question 15

Entero viruses are resistant to?

Answer 15

Phenol and chlorination

Question 16

Does disinfection kills the viruses

Answer 16

Oxidising agents of chlorine ,iodine and h2o2 kills most of the viruses

Question 17

What are inclusion bodies

Answer 17

Replicating aggregates they can be intranuclear and intrauterine cytoplasmic

Can be seen by the microscope normal

Question 18

Examples of cytoplasmic inclusion bodies

Answer 18

They are mostly eosinophilic
Negri bodies
Guarneri bodies
Paschen bodies
Bollinger bodies
Molluscum bodies-
these are mostly eosinophilic

Question 19

Negri bodies are seen in

Answer 19

Rabies

Question 20

Molluscum bodies are seen in

Answer 20

Molluscum, contagioum

Question 21

Bollinger bodies are seen in

Answer 21

Fowl pox

Question 22

Guarnieri bodies

Answer 22

Vaccinia

Question 23

Paschean bodies

Answer 23

Variola

Question 24

Intranucleur inclusion bodies

Answer 24

Cowdry type A
Herpes virus
A
Yellow fever

Cowdry type B
B
Adeno virus
Polio virus

Question 25

Both intranuclear and intra cytoplasmic inclusion bodies are seen in

Answer 25

Measles Also known as warthinfinkelday giant cells Cytomegalovirus owl appearance of infected cells

Question 26

What are the families of herpes

Answer 26

Alpha virinae Beta virinae Gamma virinae

Question 27

What are included under the alpha virinae

Answer 27

Hsv1 ( herpes simplex virus1) Hsv2( herpes simplex virus 2) Hsv3( chicken pox virus,varicella zoster) These affect the ganglion cells

Question 28

What are included under the beta virinae

Answer 28

Hhv5( cytomegalovirus) Hhv6 Hhv7 Affects the salivary glands

Question 29

What are all included under the gamma virinae

Answer 29

Hhv4 ( Epstein bar virus) Hhv8(kaposi sarcoma causing virus) Affects the B cells

Question 30

Difference between hsv1 and hsv2

Answer 30

1 causes infections above the waist 2 causes infections below the waist But there is no rule

Question 31

Describe the mucosal involvement

Answer 31

Most common site will be buccal mucosa Causes = acute gingival stomatitis Mc recurrent herpes lesions herpes labialis

Question 32

What is acute gingivo stomatitis

Answer 32

Contagious mouth infections that cause painful sores and blisters and swelling

Question 33

Most common herpes lesion involving mucosa

Answer 33

Herpes labialis

Question 34

Describe the cutaneous involvement of herpes

Answer 34

Most common site is face

Question 35

What is herpes whitlow

Answer 35

Here nurses and doctors are affected when they touch herpes patients without gloves

Question 36

Herpes gladitorum

Answer 36

Occurs in wrestling ppl

Question 37

How the herpes effects the cns

Answer 37

Temporal lobe encephalitis | Mollarets meningitis

Question 38

How herpessimplex virus | effects the eye

Answer 38

Acute dendritic ulcers are formed Steroids are contraindicated Topical acyclovir is prescribed

Question 39

Genital involvement of herpes simplex virus

Answer 39

Causes bilateral vesicular ,pustular erythematous lesions

Question 40

If pregnant female is hsv2 what should be done | To prevent infections to new born

Answer 40

Caesarea section done to avoid transmission to new born

Question 41

Lab diagnosis hsv1 and2

Answer 41

PCR Elisa Tzanck smear Tissue biopsy+ toludine blue We can see tzanck cells

Question 42

Portal of entry of varicella zoster

Answer 42

Respiratory tract/conjuctiva

Question 43

What is the secondary attack rate

Answer 43

>90%

Question 44

What is the infective period

Answer 44

2 days before the rash and 5 days after the scub falls off

Question 45

What kind of the rash Is

Answer 45

Centripetal rash and dew drop appearance and pleomorphism is seen

Question 46

What is the incubation period

Answer 46

14 -16 days

Question 47

Most common complications

Answer 47

Secondary bacterial infections

Question 48

Most serious complications of chicken pox

Answer 48

Varicella pneumonia

Question 49

Congenital varicella syndrome

Answer 49

Limb hypoplasia Microcephalic Chorizo retinitis Cicatrising lesions Vaccine strain used is oka strain

Question 50

Lab diagnosis of chicken pox

Answer 50

PCR ,Elisa | Tzanck smear

Question 51

What is the reactivation of chicken pox

Answer 51

Occurs in stress and herpes zoster is the reactivation amd also called shingles Types Herpes zoster oticus/ Ramsay hunt syndrome Bell’s palsy Vesicles over external auditory canal ,tympanic membrane and cheeks Ganglion involves genicualte ganglion

Question 52

Explain herpes zoster opthalmicus

Answer 52

Single sided lesion on the face Dera to all involvement of T3-L3 Ophthalmic branch of trigeminal nerve is involved Lab diagnosis is pcr and Elisa

Question 53

Cytomegalovirus

Answer 53

Largest herpes virus | Owl eye appearance of infected cells

Question 54

Congenital cytomegalovirus syndrome

Answer 54

Hepatosplenomegaly Microcephali Mental retardation Convulsions Calcifications Chorio retinitis Petechia lesions

Question 55

What is mononucleosis like syndrome

Answer 55

Atypically lymphocytes are seen Paul Brunel test negative Causes CMV pneumonia in post kidney transplant, post bone marrow transplant So post transplant 1-4mnths are more vulnerable to develop CMV

Question 56

Lab diagnosis of CMV

Answer 56

Culture on human fibroblasts cell line PCR Elisa

Question 57

In hiv patients thecmv

Answer 57

Causes CMV retinitis when CD count is less than 50