Coccidian Parasites Flashcards
Laboratory diagnosis of coccidian parasites r sample collection concentration technique
3 stool samples must be collected but in hiv patients. Sputum bronchial wash duodenal or jejuna aspirate can be collected..
Croncerntration sheathers sugar floating or saturation salt floatation — sedimentation technical formalin ether technique is used
Explain acid fast staining in coccidian parasites
They are stained by modified and fast staining 0.5-1 % sulphusicacid or acid alcohol kin youn method — sensitivity is low
What is gold standard test for cryptosporidium
Direct florscent antibody staining 10 times more sensitive than acid fast staining .
Oocysts of cyclospora fluorescence when viewed under epiflourscence cyst also show but not consistent
phase contrast microscopy also helpful
Features of oocyst of coccidian parasites
Cryptosporidium : 4-6 mu m 4 sporozoites
Cyclospora: 8-10 mum 2 sporozoites
cystoisospora:, 20-30 mum 4 sporozoites