Coccidian Parasites

Question 1

Laboratory diagnosis of coccidian parasites r sample collection concentration technique

Answer 1

3 stool samples must be collected but in hiv patients. Sputum bronchial wash duodenal or jejuna aspirate can be collected..

Croncerntration sheathers sugar floating or saturation salt floatation — sedimentation technical formalin ether technique is used

Question 2

Explain acid fast staining in coccidian parasites

Answer 2

They are stained by modified and fast staining 0.5-1 % sulphusicacid or acid alcohol kin youn method — sensitivity is low

Question 3

What is gold standard test for cryptosporidium

Answer 3

Direct florscent antibody staining 10 times more sensitive than acid fast staining .

Oocysts of cyclospora fluorescence when viewed under epiflourscence cyst also show but not consistent

phase contrast microscopy also helpful

Question 4

Features of oocyst of coccidian parasites

Answer 4

Cryptosporidium : 4-6 mu m 4 sporozoites

Cyclospora: 8-10 mum 2 sporozoites

cystoisospora:, 20-30 mum 4 sporozoites