VetLab (Urinalysis/Parasitology) Flashcards
In urinalysis, what are the methods of sample collection?
Free catch
Bladder expression
Catheterization
Cystocentesis
Which among the sample collection of urine is the easiest to collect and considered as the routine sample collection?
Free catch
This method of urine sample collection is done for small animals that requires palpation of bladder?
Bladder expression
In bladder expression, what is the position of the animal?
Lateral recumbancy
What is the disadvantage of bladder expression?
Possibility of insufficient urine sample
What are the materials needed for catheterization?
Sterile catheter, sterile polythropeline, lubricant, gloves
This is used in female animals in catheterization for easier visualization of urethral opening?
Speculum
What is the advantage of catheterization?
Can still collect urine sample even if the volume is low
What is the counterpart of cystocentesis in humans?
Suprapubic aspiration
Cystocentesis is used for animals who are ___________
quiet or easily restrained
What is the position of the animal in cystocentesis?
Dorsal recumbency
Which method of urine sample collection also requires palpation of bladder aside from bladder expression?
Cystocentesis
This is used in cystocentesis to visualize bladder and know where to puncture the needle?
ultrasound
What is the antiseptic used for cleansing the site in cystocentesis?
70% alcohol
What is the gauge and bore size of the needle for cystocentesis?
22-25G w/ 1 to 1 1/2 bore
In female dogs/cats, the needle is inserted in _________________ while in male dogs/cats is in ________________
midline-caudal abdomen
caudal to umbilical
Which type of collection is suitable for bacterial culture?
Cystocentesis and catheterization only
What are the other disadvantages of bladder expression?
trauma/rupture
unsuitable for culture
not recommended for animals w/ suspected obstruction or fragile bladders
Possible tissue or organ damage and difficult to perform in female animals are a disadvantage of ________________
catheterization
What is a good container for urine sample in dogs?
leak proof, w/ tight lid, opaque and clean
What is a good container for urine sample in cats?
non-absorbent litter
Examples of container collection?
Aquarium gravel, styrofoam chip
Fresh urine sample must be examined within ______________
20mins after collection
Which preservative can preserve urine for 6hrs, but specific gravity may be compromised and may cause formation of crystals?
Refrigeration
100mL of urine requires _________ of toluene
2mL
Toluene cannot _______________
prevent growth of bacteria
1 drop of formalin is adequate for _____________ of urine
up to 30mL
Formalin (40%) is used as a preservative for __________ and it prevents ____________, preserve ____________. BUt this may interfere ____________ that uses _____________
sediment analysis
bacterial/microbial growth
cellular integrity
chemical analysis
enzymatic reaction
_____________ can inhibit ________ and is appropriate preservative for ______________. But not used when you want to check pH and for chemical analysis
Boric acid (0.8%)
bacterial growth
sediment analysis
Commercial preservation tablets can control pH up to ____________ and is used as preservative for urine ____________
5 days
sediment (crystals, etc)
Refrigeration precipitates ______________
amorphous
Which preservative is not suitable for sediment and hormonal analysis?
Freezing
This preservative floats in the surface of specimen
Toluene
Unpreserved urine can result to _______________
difference in turbidity, INC bacteria, formation of crystals, and INC ammonia
What is the normal daily urine output rate?
1mL/kg/h