VESICULAR TRAFFICKING 2 Flashcards
HOW IS INACTIVE GDP CONVERTED TO ACTIVE GTP
BY GUANINE NUCLEOTIDE EXCHANGE FACTORS
HOW IS ACTIVE GTP CONVERTED TO INACTIVE GDP
BY GTPASE ACTIVATING PROTEINS
WHERE IS GDP USUALLY FOUND
IN CYTOSOL
WHERE IS GTP USUALLY FOUND
MEMBRANE ASSOCIATED
GIVE AN EXAMPLE OF A GTPASEE
RAS
WHAT ARE THE COMPONENTS OF COPII
GTPASE: SAR1
ADAPTOR: SEC23/24
COAT: SEC13/31
WHAT IS THE PROCESS OF COPII TRANSLOCATION
- THE SIGNAL OF THE CARGO WILL ENABLE THE EXIT RECEPTOR TO RECRUIT ADAPTOR PROTEINS
- EXIT RECEPTORS LINK THE CARGO WITH THE COPII COAT
- IN ORDER TO IDENTIFY THE ACTIVE SITE, SAR1 MUST BE IN ITS ACTIVE FORM
- ADAPTOR PROTEINS RECOGNISE AND SELECT CARGO ENSURING SPECIFICITY
- THE COAT PROTEINS SEC13/31 FACILITATE THE PINCHING OFF OF THE BUD
WHAT ARE THE MINIMUM REQUIREMENTS FOR COPII FORMATION
CYTOSOL (GEF, ADAPTOR PROTEINS AND COAT)
ATP
GTP
HOW WERE THE MINIMUM REQUIREMENTS FOR COPII FORMATION FOUND
RECONSTITUTION ASSAY REMOVING MEMBRANE COMPONENTS.
ONLY HAD MEMBRANE PROTEIN CONTAINING RIBOPHORIN, A RESIDENT ER PROTIEN AND P58 A COPII CARGO PROTEIN. FOUND COPII VESICLE COULD BE FORMED ONLY BY ADDING CYTOSOL, ATP AND GTP.
CYTOSOL WAS FURTHER FRACTIONED TO FIND THE IMPORTANT COMPONENT OF GEF, ADAPTOR PROTEINS AND COAT.
WHAT IS THE EFFECT OF MUTATIONS IN GTPASES
CONTITUITIVELY ACTIVE
WHAT IS THE EFFECT OF A GDP MUTANT
SEQUESTER GEF
WHAT IS THE EFFECT OF A FTP MUTANT
CANNOT HYDROLYSE GTP
TRANSPORT THROUGH THE …………………. PATHWAY IS MEDIATED VIA …………………….. VESICLES AND MICROTUBULE ……………………
SECRETORY
COATED
TRACKS
WHAT IS CRANIO-LENTICULO-SUTURAL DYSPLASIA
AUTOSOMAL RECESSIVE DEFECT IN SEC23A
LARGE DILATIONS OF ER MUTANT CELLS
COPII COATED VESICLE FORMATION AFFCETED BY SEC23A F382L
NOT ALL CARGO IS AFFECTED, SOME CARGOS INVOLVING PROCOLLAGENS ARE MORE AFFECTED.
WHY ARE NOT ALL TISSUES AFFECTED IN CRANIO LENTICULO SUTURAL DYSPLASIA
A PARALOGUE OF SEC23A , SEC23B, CAN COMPENSATE
