VENIPUNCTURE Flashcards
EQUIPMENT FOR VENIPUNCTURE
-Tourniquet
-Collection Tubes
-Additives in Collection Tubes
-Needles
-Needle Holders
-Winged Blood Collection Set (Butterfly)
-Syringes
-Solutions for Skin Antisepsis
used to provide a barrier against venous blood flow to help locate a vein
Torniquet
Additives in Collection Tubes
-Clot activators
-Anticoagulants
-Antiglycolytic Agent
-Separator Gel
Blood specimens for serum testing must first be allowed to clot for
30 to 60 minutes prior to centrifugation and removal of the serum
Accelerates the clotting
process and decreases the specimen preparation time
Clot activators
Examples of clot activators
-glass
-silica particles
-thrombin
Prevents blood from clotting
anticoagulant
Anticoagulants
-Ethylenediaminetetraacetic acid
-citrate
-oxalate
-heparin
Inhibits the metabolism of glucose by blood cells
antiglycolytic agent
The most commonly used antiglycolytic agent
Sodium fluoride
Tubes containing sodium fluoride alone yields what?
Serum
Tubes containing sodium fluoride and an anticoagulant yields what?
Plasma
An inert material that undergoes a temporary change in viscosity during the centrifugation process; this enables it to serve as a separation barrier between the liquid (serum or plasma) and cells.
Separator gel
Prevents blood from dripping into the holder when changing tubes
Rubber sleeve
The gauge number of a needle is directly related to the bore size
False,directly—-Inversely
The most common needle size for adult venipuncture
21 gauge with a length of 1 inch
Advantage of using a 1-inch needle
Provides better control during venipuncture.
Gold top additive
Clot activator and gel
for serum separation
Light Green additive
Lithium heparin and gel for plasma separation
Red top additive
-Silicone coated (glass)
-Clot activator, Silicone
coated (plastic)
Orange top additive
Thrombin-based clot activator with gel for serum separation
Royal Blue top
-Clot activator (plastic serum)
-K2EDTA (plastic)
Green top additive
-Sodium heparin
-Lithium heparin
Gray top additive
-Potassium oxalate/ sodium fluoride
-Sodium fluoride/Na2 EDTA
-Sodium fluoride (serum tube)
Tan top additive
-K2EDTA (plastic)
Lavender top additive
-Liquid K3EDTA (glass)
-Spray-coated K2EDTA
(plastic)
White top additive
-K2EDTA and gel for plasma separation
Pink top additive
-Spray-coated K2EDTA (plastic)
Light Blue additive
-Buffered sodium citrate
0.105 M (3.2%) glass
0.109 M (3.2%) plastic
-Citrate, theophylline,
adenosine, dipyridamole
(CTAD)
Gold top tube
- serum determinations in chemistry
-used for routine blood donor screening and diagnostic testing of serum for infectious disease
Light Green tube
- plasma determinations in chemistry
Red top tube
- serum determinations in chemistry
- used for routine blood donor screening and diagnostic testing of serum for infectious disease
Orange top tube
- Stat serum determinations in chemistry.
Royal Blue top
For trace-element, toxicology, and
nutritional-chemistry determinations
Green top tube
For plasma determinations in chemistry
Gray top tube
For glucose determinations
Tan top tube
For lead determinations. This tube is certified to contain less than .01 mg/mL(ppm) lead.
Lavender top tube
-K2EDTA and K3EDTA for whole blood
hematology determinations
-K2EDTA may be used for routine immunohematology testing, and blood donor screening
White top tube
For use in molecular diagnostic test methods
Pink top tube
- For whole blood hematology determinations.
- Ued for routine immunohematology
testing and blood donor screening - Designed with special cross-match label for patient information required by the AABB
Light blue
- ## For coagulation determinations
For selected platelet function assays and routine coagulation determination.
-Citrate, theophylline,
adenosine, dipyridamole (CTAD)
Order of Draw for venipuncture
- CCSHES
Culture, Coagulation, Serum, Heparin, Edta, Sodium
