HISTOPATH MTAP Flashcards
Branch of anatomical pathology that deals on the study of diseases in a given tissue or organ
HISTOPATHOLOGY
Includes the preparation, processing and staining of tissue sections for microscopic study to be interpreted by the pathologist
HISTOTECHNIQUES
Can cause death
MALIGNANT
CANNOT CAUSE DEATH
BENIGN
FUNDAMENTALS ATHOLOGY
F - Forensic
I - Immunohisto
S - Surgical
C - Cytology
A - Autopsy
M - Molecular Biopsy
COMPANY FOR RECORD KEEPING AND SPECIMEN RETENTION
COLLEGE OF AMERICAN PATHOLOGISTS
o Specimen
1 month – 1 year
o Tissue blocks
3 years – 10 years
o Slides
Indefinite
o Records
Permanent
- Preparation for organisms
WHOLE MOUNT
Result forms
TRIPLICATE
WHOLE MOUNT THICKNESS
0.2-0.5 mm thickness
- Majority of preparation
SECTIONING
SECTIONING THICKNESS
3-5 mm
Derived from blood, bone marrow and bodily fluids
SMEARING
For cytological examination
SMEARING
Microscopic examination of cells from different BODY SITES for diagnostic purposes
DIAGNOSTIC CYTOLOGY
Microscopic study of cells that have been DESQUAMATED from epithelial surfaces
EXFOLIATIVE CYTOLOGY
DERIVATIVES OF DIAGNOSTIC
Exfoliative Cytology and Fine Needle Aspiration
DERIVATIVES OF EXFOLIATIVE
Epithelial & Mucous membrane
Observation of physiological processes
FRESH TISSUE EXAMINATIONS
Also referred to as “Dissection”/”Separation”
. TEASING/DISSOCIATION
Examine as stained preparation
Bright field/Light Microscope
Unstained preparation
Phase-Contrast Microscope
- Used to locate the presence & position of mineral elements in the tissue
MICROINCINERATION
- A polarizing microscope is often used to examine the ash residue of the incinerated section
SPODOGRAM
- It determines the relationship and location of the isotopes and cells to be studied
AUTORADIOGRAPHY
Is the process in adopting to kill, harden and preserve tissue materials (from destruction and post-mortem changes
FIXATION
FACTORS AFFECTING FIXATION/PRESERVATION
- Size and nature/type of tissue
- Volume of tissue: 1:20x volume of tissue
- pH: 6-8
- Temperature: Room Temperature (20-22OC)
- Osmolality: slightly hypertonic solution
PHYSIOLOGICAL PROCESS
-MITOSIS
-PHAGOCYTOSIS
-PINOCYTOSIS
FORMALDEHYDE (FIXATES WHAT?)
FMG
FATS, MUCIN, GLYCOGEN
Most widely used for Electron Microscopy
GLUTARALDEHYDE
LEAD FIXATIVE ( FIXATES WHAT)
ACID MUCOPOLYSACCHARIDE
NEWCOMER’S FLUID ( FIXATES WHAT?)
mucopolysaccharide; nuclear proteins
ZENKER’S FLUID ( FIXATES WHAT?)
CLSN
-connective tissue, liver, spleen, nuclei
CARNOY’S FLUID ( FIXATES WHAT?)
CLUB
- chromosomes, lymph glands, urgent biopsies
- MOST RAPID
