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STUDY STUDY STUDY!!! > ENTEROBACTERIACEAE > Flashcards

ENTEROBACTERIACEAE Flashcards

1
Q

Most species are normal flora of GI Tract such
E. coli except

A

YSS - Yesernia, Salmonella, Shigella

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2
Q

Enterobacteriaceae are

A

Non – sporulating, facultative anaerobes (able
to survive with or without O2)

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3
Q

All are catalase (+) except

A

Shigella dysenteriae

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4
Q

Most are Cytochrome C oxidase negative
which separates enteric from oxidase positive
bacteria of genera

A

▪ Pseudomonas
▪ Aeromonas
▪ Vibrio
▪ Alcaligenes
▪ Achromobacter
▪ Flavibacterium
▪ Cardiobacterium

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5
Q

O antigen

A

Outer Membrane

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6
Q

H antigen

A

Flagella

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7
Q

K antigen

A

Capsule

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8
Q

Vi antigen

A

Capsule of Salmonella

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9
Q

German word meaning nonspreading

A

Ohne hauch

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10
Q

Among the E. coli, certain O antigens are
associated with specific virulent phenotype:

A

▪E. coli O 111 and E. coli O 125 are found
to cause infantile diarrhea

▪ E. coli O112 is often an invasive strain
that causes dysentery – like syndrome

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11
Q

HEAT STABLE and associated with lipopolysaccharide or endotoxin moiety of the cell wall

A

O – somatic antigen

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12
Q

From German word “Kapsel”

A

K – envelope or capsular antigen

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13
Q

A polysaccharide and Heat Stable

A

K – envelope or capsular antigen

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14
Q

K – envelope or capsular antigen is produced by?

A

KES - Klebsiella, E. coli, Salmonella

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15
Q

From German word meaning spreading

A

Hauch

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16
Q

Heat Labile & Protein in nature

A

H – Flagellar antigen

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17
Q

Enterobacteriaceae are further classified based on their

A
  1. Habitat
  2. Tribes
  3. Lactose fermentation
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18
Q

Coliforms in the Normal Flora – found In GI
TRACT

A

CHEEKS - Citrobacter, Hafnia, E. coli, Enterobacter, Klebsiella, Serratia

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19
Q

Non coliform in Normal Flora – Non-gramnegative bacilli found in GI Tract

A

MEPP - Morganella, Edwardsiella, Proteus, Providencia

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20
Q

True Enteric Pathogens – can cause disease in
GIT

A

YSS - Yersinia , Salmonella, Shigella

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21
Q

True Non – Enteric Pathogens

A

Yersinia Pestis - causes Bubonic
Plague

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22
Q

LACTOSE FERMENTERS, Appears pink (+) in MAC

A

CEEK - Citrobacter, Escherichia, Enterobacter, Klebsiella

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23
Q

NON – LACTOSE FERMENTERS

A

ShYPS - Shigella, Yersinia, Proteus, Salmonella

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24
Q

NON – LACTOSE FERMENTERS are further based on?

A

MOTILITY and H2S Production

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25
Q

Nonmotile, Non – H2s Producers

A

Shigella, Yersinia

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26
Q

Motile, H2s Producers

A

Proteus, Salmonella

27
Q

is the degradation product of amino acid Tryptophan together with pyruvic acid and ammonia

A

Indole test

28
Q

Indole is the degradation product of amino acid
Tryptophan together with pyruvic acid and
ammonia due to enzyme?

A

Tryptophanase

29
Q

KOVAC’S reagent contain

A

PDAB - Paradimethylaminobenzaldehyde

30
Q

Detects rapid indole producing organism

A

Spot Indole Test

31
Q

Requiring overnight incubation which identifies weak indole producing organisms

A

Conventional Tube Method

32
Q

Indole (+) organisms are

A

PEMPCK - P. vulgaris, E.coli, M. morganii, Providencia, C. koseri, K. oxytoca,

33
Q

3 COMMONLY USED AGAR MEDIUM

A
  1. SULFIDE – INDOLE – MOTILITY (SIM MEDIUM)
  2. MOTILITY – INDOLE – UREASE (MIU) MEDIUM
  3. MOTILITY – INDOLE – ORNITHINE (MIO) MEDIUM
34
Q

Methyl Red (+) organisms are:

A
  1. Echerichia coli
  2. Shigella species
  3. Salmonella species
  4. Citrobacter species
  5. Proteus species
  6. Yersinia species
35
Q

Methyl Red (-) organisms are:

A

KESH -
1. Klebsiella pneumoniae
2. Enterobacter species
3. Serratia marcescens
4. Hafnia species

36
Q

added as a catalyst or color intensifier to VP test

A

Alpha – naphthol

37
Q

acetoin is oxidized to

A

diacetyl

38
Q

Test should not be read after standing for over
1 hour because (-) VP cultures may produce a?

A

copper like color, potentially resulting in false (+) interpretation.

39
Q

VP POSITIVE ORGANISMS

A

KESH
1. Klebsiella species
2. Enterobacter species
3. Serratia species
4. Hafnia species

40
Q

sole carbon source

A

sodium citrate

41
Q

sole nitrogen source.

A

ammonium salts

42
Q

Product of indole test

A

Indole + Pyruvic acid + ammonia

43
Q

pH indicator of sodium citrate

A

bromthymol blue

44
Q

is an alternative test medium, which incorporates phenol red

A

Christensen’s medium

45
Q

The alkaline carbonates and bicarbonates produced as by products of citrate catabolism raise the pH of the medium to?

A

to above 7.6, causing the bromothymol blue to change from the original green color to blue

46
Q

Under alkaline conditions, pyruvate is metabolized to

A

acetate and formate.

47
Q

At pH 7.0 and below, produced what?

A

lactate and acetoin

48
Q

CITRATE NEGATIVE ORGANISMS

A

MESSSY (triple s)
Morganella, E. coli, Shigella, Salmonella typhi, Salmonella paratyphi A, Yersinia

49
Q

CITRATE VARIABLE ORGANISMS

A

PVV - P. vulgaris, Vibrio cholerae, V. parahaemolyticus

50
Q

Added to detect the production of hydrogen sulfide gas

A

Ferrous sulfate and Sodium thiosulfate

51
Q

indicator of H2S formation

A

Iron (ferrous sulfate)

52
Q

Acts as a source of nitrogen on TSI

A

peptone

53
Q

A/A, Produces gas

A

EKE- E. coli, Klebsiella, Enterobacter

54
Q

K/A, Produces gas, H2S positive

A

SPACE - Salmonella, Proteus, Arizona, Citrobacter, Edwardsiella

55
Q

K/A

A

Shigella

56
Q

K/K

A

Pseudomonas

57
Q

can be determined by microscopic examination of bacteria or by observing growth in a semi solid medium

A

Motility

58
Q

typically give diffuse, hazy growths that spread throughout the medium rendering it slightly opaque

A

Motile bacteria

59
Q

Delayed positive organisms (E.g., Enterobacter) will no produce a positive reaction due to

A

high buffering capacity of this medium

60
Q

Used for the presumptive evidence of the presence
of Helicobacter pylori in tissue biopsy material.

A

DIAGNOSTIC USE OF UREASE TEST

61
Q

A positive urease test is considered presence of

A

Helicobacter pylori

62
Q

UREASE POSITIVE

A

PUNCH : Proteus, Ureaplasma Nocardia, Cryptococcus, Helicobacter pylori

63
Q

OXIDASE POSITIVE

A

PVNCH: Pseudomonas, Vibrio, Neisseria, Campylobacter, Helicobacter

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