BACTE MTAP Flashcards
Obtain during acute phase of infection within how many days for viruses?
2-3 days
Deliver to lab within how many minutes of collection?
30 minutes
Specimens should be transported to the laboratory within how many hours of collection
2 hours
Microorganism that is susceptible to environmental conditions such as TEMPERATURE?
Neisseria meningitidis
Microorganism that is susceptible to environmental conditions such as pH?
Shigella
Specimens for VIRUSES, CHLAMYDIA, and MYCOPLASMA are usually submitted in appropriate transport media at what temperature to stabilize respective microorganisms?
4 degrees celsius
What is the transport medium for bacterial and viruses?
STUART MEDIUM
Risk group that AGENTS NOT ASSOCIATED with disease in healthy adult humans.
Risk group 1
Risk group that agents associated with human disease that is RARELY SERIOUS and for which preventive or therapeutic interventions are often available.
Risk Group 2
Agents associated with SERIOUS OR LETHAL human disease for which preventive or therapeutic interventions MAY BE AVAILABLE
RISK GROUP 3
Agents likely to cause serious or lethal human disease for which preventive or therapeutic interventions are NOT USUALLY AVAILABLE
RISK GROUP 4
Exhaust system that removes particles equal to, or greater than 0.3 microns, which essentially include all bacteria, spore and viruses, with an efficiency of 99.97%. HEPA filters are effective at trapping particulates.
HEPA (High Efficiency Particulate Air) filter
A hood with high-efficiency particulate air (HEPA) filters that provides personnel, environmental and/or product protection when appropriate practices and procedures are followed.
BSC (Biological Safety Cabinet) or biosafety cabinet
IMPROPER SWAB (CALCIUM ALGINATE)
VIRUSES
IMPROPER SWAB (WOOD)
CHLAMYDIA
WHAT ARE THE GROWTH INHIBITOR OF BACTERIA?
FATTY ACIDS
IMPROPER SWAB (COTTON SWAB)
NEISSERIA, BARTONELLA , SOME ANAEROBIC BACTERIA
Basic Media
- Nutrient Broth
- Nutrient Agar
- Saboraud’s Broth
Enriched Media (Solid)
- Blood Agar
- Chocolate Agar
Selective Media
MacConkey’s Agar
Enrichment Media (Liquid)
Selenite Broth
Differential Media
Blood Agar
Medium for Anaerobic spp.
Thiogelycollate
Media for Mycobacteria
The Lowenstein-Jensen medium
Appearance: Brown, granular colonies “buff”, rough and tough
Media for FUNGI
- Sabouraud dextrose
- Malt extract
- Brain heart infusion medium
What is added to prevent contamination of the medium by bacteria
Chloramphenicol
Examples of CHROMOGENIC MEDIA
- chromAgar
- Mannitol Agar
Quantitative ( INOCULATION OF MEDIA )
URINE CULTURES
SEMI-QUANTITAIVE (INOCULATION OF MEDIA )
PUS CULTURES
INCUBATION TEMP. FOR FUNGI
28◦C to 30◦C
INCUBATION TIME FOR BACTERIA, VIRUSES AND ACID FAST BACILLUS
35◦C to 37◦C
Anaerobe jars, bags, or chambers is composed of?
-5% to 10% hydrogen (H2),
-5% to 10% CO2
-80-90% nitrogen (N2),
-0% O2.