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BACTE MTAP Flashcards

1
Q

Obtain during acute phase of infection within how many days for viruses?

A

2-3 days

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2
Q

Deliver to lab within how many minutes of collection?

A

30 minutes

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3
Q

Specimens should be transported to the laboratory within how many hours of collection

A

2 hours

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4
Q

Microorganism that is susceptible to environmental conditions such as TEMPERATURE?

A

Neisseria meningitidis

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5
Q

Microorganism that is susceptible to environmental conditions such as pH?

A

Shigella

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6
Q

Specimens for VIRUSES, CHLAMYDIA, and MYCOPLASMA are usually submitted in appropriate transport media at what temperature to stabilize respective microorganisms?

A

4 degrees celsius

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7
Q

What is the transport medium for bacterial and viruses?

A

STUART MEDIUM

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8
Q

Risk group that AGENTS NOT ASSOCIATED with disease in healthy adult humans.

A

Risk group 1

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8
Q

Risk group that agents associated with human disease that is RARELY SERIOUS and for which preventive or therapeutic interventions are often available.

A

Risk Group 2

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9
Q

Agents associated with SERIOUS OR LETHAL human disease for which preventive or therapeutic interventions MAY BE AVAILABLE

A

RISK GROUP 3

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10
Q

Agents likely to cause serious or lethal human disease for which preventive or therapeutic interventions are NOT USUALLY AVAILABLE

A

RISK GROUP 4

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11
Q

Exhaust system that removes particles equal to, or greater than 0.3 microns, which essentially include all bacteria, spore and viruses, with an efficiency of 99.97%. HEPA filters are effective at trapping particulates.

A

HEPA (High Efficiency Particulate Air) filter

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12
Q

A hood with high-efficiency particulate air (HEPA) filters that provides personnel, environmental and/or product protection when appropriate practices and procedures are followed.

A

BSC (Biological Safety Cabinet) or biosafety cabinet

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13
Q

IMPROPER SWAB (CALCIUM ALGINATE)

A

VIRUSES

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14
Q
A
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15
Q

IMPROPER SWAB (WOOD)

A

CHLAMYDIA

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16
Q

WHAT ARE THE GROWTH INHIBITOR OF BACTERIA?

A

FATTY ACIDS

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16
Q

IMPROPER SWAB (COTTON SWAB)

A

NEISSERIA, BARTONELLA , SOME ANAEROBIC BACTERIA

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17
Q

Basic Media

A
  • Nutrient Broth
  • Nutrient Agar
  • Saboraud’s Broth
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18
Q

Enriched Media (Solid)

A
  • Blood Agar
  • Chocolate Agar
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19
Q

Selective Media

A

MacConkey’s Agar

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20
Q

Enrichment Media (Liquid)

A

Selenite Broth

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21
Q

Differential Media

A

Blood Agar

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22
Q

Medium for Anaerobic spp.

A

Thiogelycollate

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23
Q

Media for Mycobacteria

A

The Lowenstein-Jensen medium
Appearance: Brown, granular colonies “buff”, rough and tough

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24
Q

Media for FUNGI

A
  • Sabouraud dextrose
  • Malt extract
  • Brain heart infusion medium
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24
Q

What is added to prevent contamination of the medium by bacteria

A

Chloramphenicol

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25
Q

Examples of CHROMOGENIC MEDIA

A
  • chromAgar
  • Mannitol Agar
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26
Q

Quantitative ( INOCULATION OF MEDIA )

A

URINE CULTURES

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26
Q

SEMI-QUANTITAIVE (INOCULATION OF MEDIA )

A

PUS CULTURES

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27
Q

INCUBATION TEMP. FOR FUNGI

A

28◦C to 30◦C

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28
Q

INCUBATION TIME FOR BACTERIA, VIRUSES AND ACID FAST BACILLUS

A

35◦C to 37◦C

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29
Q

Anaerobe jars, bags, or chambers is composed of?

A

-5% to 10% hydrogen (H2),
-5% to 10% CO2
-80-90% nitrogen (N2),
-0% O2.

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30
Q

Procedure differentiates organisms of the domain bacteria according to CELL WALL structure.

A

GRAM STAIN

31
Q

INVENTOR OF GRAM STAIN

A

HANS CHRISTIAN GRAM

32
Q

MORDANR FOR KINYOUN STAIN

33
Q

A quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans

A

Potassium hydroxide preparation or KOH prep

34
Q

Presence of fungal elements in KOH w/ Calcofluor white

A

Chalk-white or brilliant apple green color

35
Q

A dye that intercalates or binds with the nucleic acid

A

ACRIDINE ORANGE

36
Q

Selective dye that emits green fluorescence when bound to?

A

dsDNA at 520nm

36
Q

Emits orange light (ACRIDINE ORANGE)

A

-lysosomes
-bacteria
-yeast

36
Q

Red fluorescence when bound to?

A

RNA at 650nm

37
Q

Appears orange or red with yellow-green nucleus

A

Trichomonas vaginalis

38
Q

Appears pale green to yellow

A

Human epithelial and inflammatory cells and tissue debris

39
Q

Ureaplasma and Mycoplasma can be visualized on acridine orange staining but can be differentiated through?

A

Urease test

40
Q

White pinhead colony appearance

41
Q

Bacterias which has this enzyme has the ability to cause anemia

42
Q

Growth on Loeffler’s Serum Slant
(S. areus)

A

golden yellow pigment

43
Q

Growth on Loeffler’s Serum Slant
(S. citreus)

A

lemon yellow

44
Q

Growth on Loeffler’s Serum Slant
(S. albus)

A

procelain white pigment

45
Q

Inhibitor of MSA

46
Q

PINK COLONIES ON MSA

A

S. epidermidis and S. saprophyticus

47
Q

YELLOW HALO FORMATION ON MSA

48
Q

Between STAPH AND MICROCOCCUS WHO IS SUSCEPTIBLE TO LYSOSTAPHIN AND FURAZOLIDONE BUT RESISTANT TO BACITRACIN?

A

Staphylococcus

49
Q

Color indicator of Modified oxidase Test

A

Bromthynol blue

50
Q

Most definitive test to S.areus?

A

Coagulase Test

51
Q

Enzyme that can possibly dissolve clot

A

Fibrinolysin / staphylokinase

52
Q

Cell wall, anti-phagocytic, virulence of S.aureu

53
Q

1 wound infection; #1 osteomyelitis (septic arthritis)

54
Q

DNASE COLOR (POSITIVE REACTION)

A
  • Toluidine blue ( pink )
  • Methyl green ( clear )
55
Q
  • Causes endocarditis (associated with Prosthetic Heart Valve Surgery infection)
  • Novobiocin SUSCEPTIBLE
  • Non-hemolytic
  • Blood culture contaminant
A

S. epidermidis

56
Q

-Causes UTI in young women; Specimen for diagnosis: urine
- Novobiocin RESISTANT (less than 16mm)

A

S. saprophyticus

57
Q

Destroys stratum granulosum of skin

A

Exfoliatin

58
Q

1 cause of food poisoning in the Philippines

59
Q

White pinpoint colonies

A

Streptococcus

60
Q

Selective medium for Streptococcus

A

Phenyl Ethyl Alcohol Agar

61
Q

NON-ENTEROCOCCUS

A
  • S. bovis ( common cause of Colon cancer)
  • S. equinis
62
Q

Lancet or dome shape colonies

A

S. pneumoniae

63
Q
  • Leading flesh-eating bacteria (necrotizing fasciitis)
  • RHD (Rheumatic Heart Disease) / RF (Rheumatic Fever)
  • AGN (Acute Glumerulonephritis)
A

S. pyogenes

64
Q
  • # 1 neonatal meningitis
  • resistant to all ( Taxo A, P and SXT)
A

S. agalactiae

65
Q

Differentiates Group D from other Streptococcus

A

BEA (Bile Esculin Agar)

66
Q

Indicator of BEA

A

Ferric Ammonium Citrate

67
Q

Color indicator of PYR test

A

P-dimethylaminocinnamaldehyde

68
Q

Indicator of Sodium Hippurate

69
Q

Best test to identify S. pyogenes

A

Bacitracin Susceptibility Test

70
Q
  • rust-colored sputum
  • # 1 adult bacterial meningitis
  • # 1 otitis media
A

S. pneumoniae

71
Q

VIRIDANS

A

MUSCISM
- mutans
- uberis
- salivarus
- constellatus
- intermedius
- sanguis
- mitis

72
Q

Used to identify S. pneumoniae

A

Optochin Disk Test (Taxo P)
COMPOSITION: ethylhydroxycuprein HCl

73
Q

Detects pneumococcal antibody; skin test for S. pneumoniae infection

A

Francis Test

74
Q

Detects erythrogenic antigen; skin test for S. pyogenes infection

A

Dick’s test

75
Q

A skin test and immunity test for scarlet fever that uses antitoxin to the erythrogenic toxin of S. pyogenes subcutaneously

A

Schultz Charlton test

76
Q

Transport system for Neisseria

A

JEMBEC system

77
Q

Presumptive (screening) test for gram negative cocci

A

Oxidase Test / Taxo N

78
Q

3rd cause of otitis media (middle ear infection)

A

Moraxella catarrhalis

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