Vaginal thrush/candidiasis OSPE

Question 1

case

Answer 1

burning sensation and itchiness of vagina
-erythema of vaginal mucosa and thick,cheesy-white precipitates

Question 2

specimen

Answer 2

High vaginal swab

Question 3

microscopy

Answer 3

KOH mount:budding yeast cells,pseudohyphae
Gram-stain: gram + budding yeast cells with pseudohyphae

Question 4

culture

Answer 4

media: SDA,Sheep blood agar, brain heart infusion agar
colony: smooth,creamy colonies,yeasty odour

Question 5

identification of isolates (species) ***

Answer 5

1.germ tube test:elongated tubes (+) = to identify C.albicans

2.Chlamydospores formation on nutritionally deficient media: corn meal agar, rice starch agar = to identify C.albicans

3. Carbohydrate fermentation test
4. Carbohydrate assimilation test

4.Chrom Agar

Question 6

serology/ biochem

Answer 6

Ab detection: LA.ELISA

Ag detection: Elisa-Mannan,cytoplasmic Ag

detection of fungal metabolites: G-test for d-glucan

Question 7

molecular

Answer 7

PCR

Question 8

etiology

Answer 8

C.albicans
C.glabrata
C.tropicalis

Question 9

morphological type

Answer 9

yeast-like