malasezia furfur

Question 1

Samples

Answer 1

Skin scrapings from patients with superficial lesions, blood and indwelling catheter tips from patients with suspected fungaemia.

Question 2

Microscopy:

Answer 2

5-10% Potassium hydroxide (KOH) examination: “Spaghetti and meatballs appearance” of hyphae and yeast cell clusters.
* Colour contrasts with KOH : Methylene blue stain, Ink blue stain

Question 3

Culture on Sabaroud’s dextrose agar:

Answer 3

low growing ( 1 -2 weeks)
* Modified SDA supplemented with fatty acids
* Shiny and white→turns cream→becomes dull and beige

Question 4

other

Answer 4

Wood lamp (ultraviolet black light ) examination: “Yellowish-green/ Coppery- orange fluorescence”