Cryptosporidium Parvum OSPE

Question 1

SAMPLE

Answer 1

STOOL

Question 2

MICROSCOPY

Answer 2

direct wet mount :colorless, spherical oocyst of 4- 5 μm, containing large and small
granules
Modified acid-fast staining: pink oocyst

direct/ndirect immunofluorescence microscop
Fluorescent staining:auramine-phenol or acridine orange

Question 3

other

Answer 3

Enzyme immunoassay (EIA)
(PCR)

Question 4

other

Answer 4

Positive Crypto-Strips antigen detection test
Histopathological Examination: H&E staining revealed Cryptosporidia-infected human small intestine.

Question 5

case

Answer 5

veterinarian
diarrhea of 2 weeks’ durationthe diarrhea as thin, watery, and non-bloody. She reported 10 to 14 diarrheal stools per day, the frequency of which was not influenced by a variety of over-the-counter antidiarrheal medications.
negative (HIV) serologic test,
- acid-fast oocysts.

Question 6

likely source

Answer 6

zoonotic acquisition from one of her animal patients.

Question 7

If this individual were HIV positive, what other intestinal pathogens would have been considered?

Answer 7

E. histolytica, Giardia, Cystoisospora, Cyclospora, and Microsporidia

Question 8

go tru pic n questions fr lab slide

Answer 8

-