Gonorrhoea OSPE

Question 1

specimen

Answer 1

urethral swab/discharge
endocervical swab
blood culture

Question 1

case

Answer 1

• purulent discharge from his external genitalia and dysuria.
  -unprotected sex.
  -intracellular Gram-negative diplococci along with abundant polymorphs were found.

Question 2

Collection:

Answer 2

he urethral meatus is cleaned with gauze soaked in saline
* The purulent discharge is expressed out by pressing at the
base of the penis
* Collected directly on to slides or swabs
In chronic urethritis: As discharge is minimal, prostatic massage is done
to collect the secretion; alternatively, the morning drop of secretion may
be collected.

Question 3

Transport media

Answer 3

Specimen is collected in charcoal-coated swabs kept in Stuart’s
transport medium or Amies medium

Question 4

Microscopy

Answer 4

gram negative
bean shaped intracellular diplococci
- Predominantly within polymorphonuclear leukocytes in a smear

Question 5

Culture media

Answer 5

❑Selective medium:
* Thayer-Martin medium
* New York City medium
❑Non-selective: Chocolate medium
❑Modified Thayer Martin= GC agar (Gonococcus agar)+ antibiotics

Question 6

biochem

Answer 6

❑Catalase: +
❑Oxidase: +
❑Carbohydrate utilization:
* Ferment only glucose ( +ve) , but not maltose ( - ve)

Question 7

Molecular method

Answer 7

Gold standard
❑Nucleic Acid Amplification Tests (NAATs) * PCR
❑Non-Amplification Test * DNA probes