MUCORMYCOSIS

Question 1

Specimens:

Answer 1

The deep or endoscopy-guided nasal swab, paranasal sinus, or orbital tissue, using a KOH mount, PAS, GMS and calcofluor white staining methods.

Question 2

Microscopy of colonies

Answer 2

Non-septate or pauci-septate, irregular, ribbon-like Microscopic examination of a hyphae; Wide-angle of non-dichotomous branching

Lactophenol Cotton Blue staining:Sporangium liberating spores and aseptate hyphae (spore forming presence of rhizoids.

Question 3

Culture:

Answer 3

Sabaroud’s Dextrose Agar: White color to Grey color colonies.

Question 4

Molecular Assay

Answer 4

PCR, RFLP, and DNA sequencing, molecular-based diagnostic tools use an internal transcribed spacer or the 18S rRNA genes

Question 5

Serum tests

Answer 5

ELIZA, immunoblots, and immunodiffusion tests

Question 6

Hematoxylin-eosin ((H&E

Answer 6

presence of mycelial fungal filaments in this tissue sample.

Question 7

RISK

Answer 7

Diabetic
ketoacidosis and renal acidosis predispose to mucormycosis.