Vaginal Cytology and Male Repro Cytology Flashcards
4 Vaginal epithelial cells
- Basal
- Parabasal
- Intermediate
- Superficial
4 Stages of Estrous
- Proestrus
- Estrus
- Diestrus
- Anestrus
Average age of occurence of vaginal neoplasias
10 yrs
The most common form of reproductive neoplasia
Mammary tumors
Vaginitis occurs regardless of
age, breed, repro status
Oldest vaginal epithelial cells that may or may not have a nucleus
Superficial
Youngest vaginal epithelial cells
Basal
Which part of estrous cycle where the female is attractive to the male but wont stand for breeding?
Lots of RBCs are seen on smear
Proestrus
Which stage of estrous is when females will seek out males and stand for breeding?
90% of fully cornified superficial cells and bacteria
Estrus
Which stage of estrus is where the corpora lutea are present?
Increase in progesterone
Diestrus
Which stage of estrus is the prolonged period of sexual rest?
Minimal secretions, cervix is tightly constricted, and vaginal mucosa is pale
Anestrus
This occurs regardless of age,breed, or repro status
Secondary to vaginal anomalies
C/S: mucoid to purulent discharge, pollakuria, and licking of the vulva
Vaginitis
2nd most common form of reproductive neoplasia
Vaginal Neoplasia
Average age of occurrence of vaginal neoplasia
10 yrs
Procedure for collecting Female sample (4 steps)
- Part lips of the vulva, insert swab at steep 90 degree angle
- After 1-2 in, alter the angle to 45 degrees
- When swab is fully inserted, rotate 2-3 turns
- Gently withdraw
To prepare a vaginal cytology smear, you should _______ the sample on to the slide
roll
Cytologic values for Proestrus
Superficial cells are predominate Non-degen neutrophils Parabasal Intermediate Bacteria
If bred within a day of preparing a vaginal smear, what may be seen?
Sperm
Cytologic values for Estrus
90% superficial
<5% parabasal and intermediate
bacteria
Cytologic values for Diestrus
Superficial cells decrease by 20%
Intermediate cells increase
Cytologic values for Anestrus
Parabasals and intermediate cells
Low neutrophils and RBCs
Semen collection technique (6 steps)
- Grasp prepuce, and push back to expose the tip of penis
- Slide collection cone over the penis and push prepuce down to expose the bulbis glandis
- Lock fingers in a ring around penis and hold the bulbis glandis in fist
- Apply pressure with forward and backward movement (male may thrust on his own)
- Watch for semen flow into collection tube
- Continue to apply pressure until fluid is crystal clear flows into collection tube
Collection method where a female in estrus expedites semen collection
Teaser Female
Collection method to obtain semen from cattle, horses, sheep, goats, rabbits, and cats
Male mounts the female and penis is diverted into AV
Artificial vagina
This collection method is used to collect semen from dogs, pigs, chickens, and turkeys
Apply pressure and massage the penis, when animal is aroused a director cone is attached to a collection tube and slipped over the penis
Digital manipulation
This collection method to obtain semen involves low-voltage pulses of current to the pelvic nerves to have an ejaculatory response
Electroejaculation
Normal semen should be
Milky white and moderately viscous
Blood in semen would be
red/pink
Contamination in semen would be
yellow in color
If there is an infection, semen would be
green/grayish, serous
Normal semen should have __% motility
70
6 Semen morphological abnormalities
- misshapen head
- 2 heads
- detached head
- bent tail or midpiece
- coiled tail
- droplets
Method used to collect sample from testes/neoplasia
FNA
Neoplasia that occurs in older or cryptorchid males
Firmest of the testicular tumors and white-grey in color
Usually always benign
Sertoli cell tumors
Neoplasia that can cause testicular enlargement
Semi-firm with a homogenous, glistening grey to white
Produce a starry sky appearance upon cytology exam
Seminomas
Neoplasia where mestastasis is extremely rare
Soft appearance
Interstitial Cell tumors
