Cytology Flashcards

1
Q

Increase in size of an organ or in a select area of tissue

A

Hypertrophy

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2
Q

Abnormal increase in the number of cells (increase in mitosis)

A

Hyperplasia

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3
Q

The pathological process that results in the formation and growth of a tumor

A

Neoplasia

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4
Q

A tumor that can be benign or malignant

A

Neoplasm

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5
Q

The change in the type of adult cells in a tissue to a form that is abnormal for that tissue

A

Metaplasia

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6
Q

Abnormality of development; alteration in size, shape, and organization of adult cells (non-cancerous)

A

Dysplasia

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7
Q

Cancer cells that divide rapidly and bear little or no resemblance to normal cells

A

Anaplasia

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8
Q

Microscopic appearance of nuclear chromatin, the coarser the chromatin, the greater the chance of malignancy

A

Chromatin pattern

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9
Q

This sample collection technique is used to determine the stage of estrous in breeding females, evaluate uterine and vaginal dz, ear cytology, and fistulous tract (tube-like passages)

A

Swab

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10
Q

When staning and viewing a swab sample, you should use which stain and view on what power?

A

Diffquick

100x

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11
Q

This sample collection technique uses a scalpel blade with mineral oil to scrape a certain area of skin.

A

Skin scrape

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12
Q

An advantage to the skin scrape technique is? Disadvantage?

A

It collects many cells;

Only collects superficial cells from inflammation

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13
Q

When viewing a skin scrape sample, it should be stained in _______ and viewed at what power?

A

diffquick

40/100x

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14
Q

This type of slide is made from lesions and tissues removed during necropsy or surgery and is easy to collect, but only obtains a few cells

A

Touch imprint

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15
Q

This technique uses 4 slides

A

Tzanch

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16
Q

This technique is performed on masses of the lymph nodes, nodular lesions, and internal organs at several areas and depths of the mass
It’s good for cutaneous lesions, but only collects a few cells

A

FNA (fine needle aspirate)

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17
Q

2 collection methods for a FNA

A

Aspiration and non-aspiration

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18
Q

Non-aspiration is AKA

A

capillary/stab technique

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19
Q

This technique is similar to a blood smear

A

Linear/Line prep

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20
Q

This technique involves dragging the needle tip through the sample at various lengths and directions

A

Starfish

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21
Q

This technique involves using a second slide perpendicular OR parallel to the sample slide and pulling it smoothly over the sample

A

Compression (Squash)

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22
Q

This technique is similar to the compression prep except when placing the 2nd slide parallel to the sample slide, you place it perpendicular and rotate the slide 45 degrees

A

Modified Compression

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23
Q

This technique allows you to evaluate cells more thoroughly

Middle = highly concentrated with cells, 1/3 is prepared using squash, 1/3 is prepared using linear

A

Combination

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24
Q

Site is clipped, not scrubbed, clean with alcohol

Can be used to sample almost any tissue

A

Tissue Biopsy

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25
Q

2 methods of tissue biopsy

A

Wedge and punch

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26
Q

Tissue biopsy sample is obtained with a scalpel blade

Used for solitary lesions

A

Wedge

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27
Q

Tissue biopsy sample is obtained with a punch tool

Quick and easy to do

A

Punch

28
Q

Most common type of punch biopsy

A

Keyes

29
Q

Biopsy samples should be placed in _________ within one minute of obtaining and should be allowed to preserve for ___ hrs

A

formalin; 24

30
Q

You should not ________ a tissue sample

A

freeze

31
Q

Introduction of a needle into a body cavity or organ to remove fluid

A

Centesis

32
Q

Done in the peritoneal cavity

A

Abdominocentesis/parecentesis

33
Q

Done in the thoracic/pleural cavity

A

Thoracocentesis

34
Q

Done in the bladder

A

Cystocentesis

35
Q

To obtain CSF

A

CSF tap

36
Q

Taken from within a joint space

A

Arthrocentesis

37
Q

To obtain aqueous or vitreous humor

A

Eye centesis

38
Q

Cytological evaluation of mucus samples obtained from the trachea, bronchi, or bronchioles to assist with diagnosing pulmonary disease

A

Transtracheal/Bronchial wash

39
Q

3 techniques to a transtracheal/bronchial wash

A
  1. Percutaneous
  2. orotracheal
  3. bronchoalveolar
40
Q

Area is injected with lidocaine, jugular catheter is placed in the trachea and flushed with saline, when the animal coughs retract plunger and collect sample

A

Percutaneous

41
Q

Requires light anesthesia and placement of an endotracheal tube
Urinary/jugular catheter is placed through endotracheal tube and flushed with saline. Animal may not cough, so the sample should be collected after a few seconds

A

Orotracheal

42
Q

Bronchoscope is needed and samples are collected to assess lower respiratory tract

A

Bronchoalveolar

43
Q

Remember to collect ___ fluid expelled when an animal coughs and put into a red top tube

A

ALL

44
Q

This aids in the diagnosis of upper airway diseases
Normal saline is infused into the nasal cavity using a syringe and tubing; sample is aspirated
Helps diagnose inflammation secondary to sepsis, fungi, yeast, and neoplasm

A

Nasal flush

45
Q

A fluid that is colorless, low specific gravity, low protein, little to no RBCs, 95-100% lymphocytes

A

CSF

46
Q

A fluid that is colorless to straw yellow, transparent to slightly cloudy, odorless, and low TP

A

Peritoneal fluid

47
Q

A fluid that is due to a ruptured mass where blood leaks into the peritoneal cavity

A

Hemoabdomen

48
Q

A fluid that is pulled in a thoracocentesis. It is milky white and contains a large amount of fats and some lymphocytes

A

Chylous effusion

49
Q

Viscosity should be observed by performing a mucin clot test

Should be thick

A

Synovial fluid

50
Q

Large amounts of mucus are found in this fluid

A

Tracheal wash

51
Q

How many slides should be made from a fluid sample?

A

Multiple

52
Q

Fluid samples should be placed in

A

EDTA tubes

53
Q

All fluid placed on slide should remain on slide and not spread off the edge

A

Edge of Cliff syndrome

54
Q

The preferred fixative for cytological evaluation

A

95% methanol

55
Q

2 general types of stains used for cytology

A
  1. Romanowsky

2. New Methyelene blue

56
Q

3 Romanowsky stains

A
  1. Diff Quick!!!!
  2. Giemsa
  3. Wright’s
57
Q

Used as an adjunct to Romanowsky stain

A

NMB

58
Q

These are inexpensive, readily available, easy to prepare, maintain, and use

A

Romanowsky stain

59
Q

Bone marrow and lymph node samples should set in Diff quik for how long?

A

1-2 minutes

60
Q

This stain stains the cytoplasm weakly but gives excellent nucleolar details
Used for nucleated cells, most infectious agents, platelets, bacteria, fungi, yeast, and mast cell granules

A

NMB

61
Q

Detects the presence of bacteria

A

Gram stain

62
Q

Gram negative

A

red/pink stain

63
Q

Gram positive

A

purple

64
Q

Used for histological evaluations

A

Hematoxin/Eosin stain

65
Q

Commonly used with human gynecological exams because it provides good nuclear detail

A

Papanicolaou stain

66
Q

Made by placing a drop of immersion oil on the specimen, followed by a coverslip

A

Temporary mount

67
Q

Made with commercially available cover slip mounting glue

A

Permanent mount