Cytology Flashcards
Increase in size of an organ or in a select area of tissue
Hypertrophy
Abnormal increase in the number of cells (increase in mitosis)
Hyperplasia
The pathological process that results in the formation and growth of a tumor
Neoplasia
A tumor that can be benign or malignant
Neoplasm
The change in the type of adult cells in a tissue to a form that is abnormal for that tissue
Metaplasia
Abnormality of development; alteration in size, shape, and organization of adult cells (non-cancerous)
Dysplasia
Cancer cells that divide rapidly and bear little or no resemblance to normal cells
Anaplasia
Microscopic appearance of nuclear chromatin, the coarser the chromatin, the greater the chance of malignancy
Chromatin pattern
This sample collection technique is used to determine the stage of estrous in breeding females, evaluate uterine and vaginal dz, ear cytology, and fistulous tract (tube-like passages)
Swab
When staning and viewing a swab sample, you should use which stain and view on what power?
Diffquick
100x
This sample collection technique uses a scalpel blade with mineral oil to scrape a certain area of skin.
Skin scrape
An advantage to the skin scrape technique is? Disadvantage?
It collects many cells;
Only collects superficial cells from inflammation
When viewing a skin scrape sample, it should be stained in _______ and viewed at what power?
diffquick
40/100x
This type of slide is made from lesions and tissues removed during necropsy or surgery and is easy to collect, but only obtains a few cells
Touch imprint
This technique uses 4 slides
Tzanch
This technique is performed on masses of the lymph nodes, nodular lesions, and internal organs at several areas and depths of the mass
It’s good for cutaneous lesions, but only collects a few cells
FNA (fine needle aspirate)
2 collection methods for a FNA
Aspiration and non-aspiration
Non-aspiration is AKA
capillary/stab technique
This technique is similar to a blood smear
Linear/Line prep
This technique involves dragging the needle tip through the sample at various lengths and directions
Starfish
This technique involves using a second slide perpendicular OR parallel to the sample slide and pulling it smoothly over the sample
Compression (Squash)
This technique is similar to the compression prep except when placing the 2nd slide parallel to the sample slide, you place it perpendicular and rotate the slide 45 degrees
Modified Compression
This technique allows you to evaluate cells more thoroughly
Middle = highly concentrated with cells, 1/3 is prepared using squash, 1/3 is prepared using linear
Combination
Site is clipped, not scrubbed, clean with alcohol
Can be used to sample almost any tissue
Tissue Biopsy
2 methods of tissue biopsy
Wedge and punch
Tissue biopsy sample is obtained with a scalpel blade
Used for solitary lesions
Wedge