Urinalysis Pt 2 Flashcards
Sedi-stain is aka
Sternheimer-Malbin
Primary choice stain of UA
Sedi-stain
To examine crystals, you should view a slide on
10x
To examine bacteria, WBCs, and RBCs, you should view on
40x
When an animal has pyuria, there is usually this many in a slide?
> 5/hpf
Normal finding for RBCs
up to 5/HPF
This may be seen in urine from males collected by voiding, catheterization, or cystocentesis
Confirms intact male
Sperm
Cellular covering of both internal and external body surfaces
Epithelial cells
Largest in size of the epithelial cells that are shed from the distal urethra, vagina, vulva, and prepuce
Looks like a fried egg (thin, flat, angular, or irregular outline, small round nucleus)
Squamous cells
Normal # for squamous cells
Low #s
Epithelial cells that have a tail and are round
Originate from the renal pelvis, ureters, bladder, and urethra
Transitional
Smallest epithelial cells seen
Renal Tubular
Tumor cells of the urinary tract
Neoplastic cells
These form in the lumen of the distal and collecting tubules of the kidneys where the concentration and acidity of urine is the greatest
Composed of protein and mucoproteins
Casts
Normal # of casts to see upon UA
few to none
Casts dissolve in this type of urine
dilute/basic
Cast that is smooth, clear, colorless, and semi-transparent with parallel sides and clear margins and blunted ends
Composed only of protein
Seen in increased numbers with proteinuria, strenuous exercise, and general anesthesia
Hyaline
Most common cast that is seen in UA
Textured appearance that can be fine or coarse
Contain granules that were once epithelial cells, RBCs, or WBCs
Seen in large numbers with nephritis and kidney damage
Granular
These casts are wider than hyaline and have square ends
Smooth, Colorless to grey and highly refractive
Large numbers indicate degeneration of renal tubules and always pathogenic
Waxy
These casts can have any type of cell inside and their membrane may or may not be visible
Deep yellow to orange in color
Form when RBCs cluster within the lumen of the tubules
Indicate renal bleeding
Cellular (Erythrocyte)
These casts are rarely seen in dogs and contain small lipid droplets
Large numbers indicate renal tubular degeneration
Seen with Diabetes Mellitus
Most common casts in cats with renal dz
Fatty
Identified in stained or unstained urine
Rod or cocci
Bacteria
If you suspect bacteria, you could use this to diagnose
Gram stain
Identified in unstained urine
Look like snowmen or footprints, colorless, and may have budding
Represents contamination, infection, or an old sample
Yeast
Hyphae represent overgrowth of contaminants in sample where analysis was delayed
If in fresh urine, indicates fungal infx of kidney or bladder
Fungus
Fungus that can cause systemic infections including colonization of the renal pelvis
Aspergillus terreus
Can be confused with casts, but do not have defined edges, resemble a twisted ribbon
Common in horse urine
Mucus thread
Comes from dusting powders within gloves
Variable in size and have a Y-shape dot in the center
Starch
Sometimes contaminates urine samples and can include parasite eggs
Fecal material
A parasite that is typically non-pathogenic that can be seen in a urine sample
Looks similar to Tricherus
Capillaria plica
C. plica is AKA
bladder worm
Dogs and cats can become infected with C. plica by eating
an earthworm
A single dose of this drug should get rid of C. plica
Ivermectin
This type of parasite is seen due to hemorrhaging
Microfilaria (<3worm)
This parasite’s eggs are yellow/brown thick walled ova with a wavy surface
Infects cats, dogs, and humans or carnivorous animals
Dioctophyma renale
Dioctophyma renale is AKA
Giant kidney worm
Animals can become infected with D. renale by
eating raw meat
Common finding in cat urine
Refractile spheres that result from obesity, diabetes, and KY Jelly
Fat Droplets (lipiduria)
Round to oval in shape with a yellow to brown tint with variable appearance
Can be confused with parasite eggs
Mold/Pollen spores
Take on interesting forms and shapes
Should not be confused with microorganisms
Cloth Fibers
Elongated, asymmetrical and refractile appearance, not to be confused with fungus or parasite larva
hair fibers
When a coverslip is placed on a slide containing urine sediment, these can appear
air bubbles
Crystals in the urine
crystalluria
Most common crystal found in urine
Colorless and 3D appearing, look like coffin lids
Found in urine with any pH
Caused by UTI or bacteria
Struvite
Struvite is AKA
triple phosphate
Struvites prefer a more _______ to ____________ urine
neutral to alkaline
Second most common crystal found in urine
Calcium Oxalate
2 types of calcium oxalate crystals
Monohydrate and dihydrate
This type of calcium oxalate crystal can be seen with antifreeze poisoning
Spindle, oval, or dumbell shaped
Monohydrate
This type of calcium oxalate crystals look like squares than contain an x
If seen in large numbers, can be signifying antifreeze poisoning or acute renal failure
Dihydrate
This breed of dog is predisposed to Calcium Oxalate Dihydrate crystals
Mini schnauzers
This type of crystal favors acidic urine and look like stop signs found in layers
Cystine
Increased numbers of cystine crystals that could indicate renal tubular dysfunction
Cystine crystalluria
These crystals favor alkaline urine and are yellow/brown in color, spheres with spicules (kind of like an acanthocyte)
Ammonium urate/biurate
2 dog breeds that are predisposed to Ammonium urate crystals
Dalmatians and english bulldogs
Favor alkaline urine
Yellow, brown, or colorless spherical with striations or dumbbell shaped
Normal in horses, rabbits, guinea pigs, and goats
Calcium carbonate
Animals that Ca Carb crystals are normal to see in
horses, rabbits, guinea pigs, and goats
Favor acidic urine and are yellow/brownish
Spheroids with concentric striations and dense (tree ring appearance)
Rare and seen with tyrosine
Leucine
These crystals favor acidic urine and a yellow to light brown with needles protruding from the center and seen in small clusters
Rare in animals, but seen with liver dz in humans
Tyrosine
Aggregates of finely granular material without definite shape
Amorphous
These amorphous crystals are made of Na, K, Mg, or Ca salts
Form in acidic urine
Brown/yellow in color
Amorphous urate
These amorphous crystals are similar to urates but are clear and form in alkaline urine
Amorphous phosphates
These crystals are common in canine urine that is highly concentrated and precipitates onto other elements in urine
Billirubin
These crystals prefer acidic urine and are yellow/brown in color and look like a lemon or diamond shape
Uric acid
This breed of dog can’t convert uric acid so they excrete it in urine
Dalmatian
Uric acid stones tend to be more common in these dalmatians
males
Seen with animals that have been treated with sulfonamides
Resemble an iris or hay bushel and develop from decreased water consumption during treatment
Sulfonamide