URINE SEDIMENTS STAIN CHARACTERISTICS Flashcards

1
Q

This dileneates structure and contrasting colors of the nucleus and cytoplasm

A

Sternheimer - Malbin

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2
Q

The Sternheimer - Malbin functions for?

A

WBCs, ECs and Casts

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3
Q

The Sternheimer - Malbin stain is composed of?

A

Crystal violet and Safranin O

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4
Q

This enhances nuclear details

A

0.5% Toluidine BLue

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5
Q

The 0.5% Toluidine blue functions for?

A

The differentiation of WBCs and RTE cells

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6
Q

This lyses RBCs and enhances the nucleus of WBCs

A

2% acetic acid

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7
Q

What is the function of 2% acetic acid?

A

Distinguishes RBCs from WBCs, oil droplets and crystals

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8
Q

This stains the TAG And neutral fats orange - red

A

Lipid stains

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9
Q

What is the function of lipid stains?

A

Identifies free fat droplets and lipid containing cells and casts

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10
Q

Lipid stains is composed of?

A

Sudan III and ORO

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11
Q

This stain differentiates gram negative to gram positive bacteria

A

Gram Stain

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12
Q

The gram stain functions for?

A

Bacterial casts

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13
Q

This stains the eosinophilic granues

A

Hansel stain

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14
Q

The Hansel stain functions for?

A

Urinary eosinophils

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15
Q

What is the composition of Hansel stain?

A

Eosin Y and Methylene blue

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16
Q

This stains the sediments containing Iron

A

Prussian Blue

17
Q

What is the function of PRUSSIAN BLUE?

A

Identifies hemosiderin granules in casts and cells