MICROSCOPIC EXAMINATION OF URINE Flashcards
In microscopic examination, what should be the volume of the specimen that should be collected?
10 - 15mL
Why is 12 mL volume frequently used in microscopic examination?
The multiparameter reagent strips are easily immersed
Proper centrifugation of the specimen should be at?
400 RCF for 5 minutes
The usage of this mechanism to slow centrifuge causes disruption of the sediment prior to decantation and should not be used
Braking mechanism
How much volume should be should in sediment preparation?
0.5 to 1.0 mL
What should be the recommended volume when using the conventional - glass method?
22 uL or 0.02 mL
What method requires 20uL of sample?
Conventional glass - slide method
This is primarily used to monitor the course of diagnosed cases of renal disease
Addid COunt
What is the specimen used in Addis count?
12 hour urine sample
What is the preservative used in Addis count?
Sodium Fluoride
What elements has the same refractive index with urine?
Hyaline casts and mucus threads
What elements should be the first to look for in a specimen?
Epithelial cells
This is not part of the routine examination of the urine sediment
Cytodiagnostic Urine Testing
What do cytodiagnostic urine testing provides?
Definitive information about RT changes and provides additional method for detecting and moniroting renal disease
What is the specimen used in Cytodiagnostic Urine TEsting?
First morning urine
What is the stain used in Cytodiagnostic Urine Testing?
Papanicolaou stain
This will happen to concentrated urine as the cells will shrink due to loss of water and may appear crenated or irregularly shaped RBCs
Hypersthenuric
Diuted urine is often called?
Ghost cells
What do you call a diluted urine?
Hyposthenuria
RBCs varies in size, have cellular protrusions or are fragmented
Dysmorphic RBCs
Dysmorphic RBCs is associated with?
Glomerular bleeding
The macroscopic hematuria is frequently associated with?
Advanced glomerular damage
This is also seen with damage to the vascular integrity of the urinary tract
Macroscopic hematuria
This predominant WBC found in the urine sediment
Neutrophils