Unit 6: Flashcards
Define polymor
One single unit that’s made up of monomers
Monomers
Building blocks linked together to form a polymor
What is DNA made up
Nucleotides are the monomer
And polymore
(The monomer for nucleic acids is nucleotides
List the three parts of a nucleotide
Phosphate
Deoxyribose (5 carbon atoms)
Nitrogenous
List the four types of nitrogenous
Adenine
Cystosine
Guanine
Thymine
List the two patterns found between nitrogenous
Adenine and Thymine go together
Cytosine and Guanine go together
How does DNA replicate
- An enzyme helicose unwinds and unzip the DNA
- Anneal (prime) the DNA with the enzyme Primase
- Build new strands between the bonds
- Lymose brings the backbone tofether
Steps for PCR
- Unzip DNA with heat to replace helicase
- Artificial primers to anneal
- Build DNA with TAQ polymerase
DNA restriction pattern
GAATTC
CTTAAG
What’s the direction of DNA strands
5 prime to 3 prime
Or
3 prime to 5 prime
- Why does lymose fill in the breaks
Because polymerase only builds new strands in 5 prime to 3 prime resulting in Okazaki fragments. This strand is called the lagging strand
- What is primer made out of ?
RNA
- How do you identify a person with DNA
Take a gel electrophoresis from a crime scene and then take millions of different peoples DNA and compare them to find the exact combination
- How do DNA strands move in a gel electrophoresis
Longer strands stay near the wells while shorter strands move more away
What do all amino acids have
H3N and CH