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Microbiology > Unit 4: Aerobic GPB > Flashcards

Unit 4: Aerobic GPB Flashcards

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Biology 101 flashcards
1
Q

Arcanobacterium haemolyticum: clinical significance?

A
  • Acute pharyngitis similar to Group A strep

- Rash in 50%

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2
Q

Arcanobacterium haemolyticum: Important tests

A
  • Reverse CAMP

- Lecithinase POS

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3
Q

Arcanobacterium haemolyticum: colony morphology

A

Small beta

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4
Q

Bacillus anthracis: colony morphology

A

“medusa head” “egg white” nonhemolytic

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5
Q

Bacillus anthracis: Gram stain

A

Large GPB; boxcar

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6
Q

Bacillus anthracis: clinical significance?

A
  • Potential bioterrorist agent
  • 3 Forms
  • Reservoir: herbivorous animals and spores in soil
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7
Q

Bacillus anthracis: Important tests

A
  • Egg yolk agar POS
  • No growth on PEA
  • Lecithinase POS
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8
Q

Bacillus cereus: colony morphology

A

Green, feathery spreading and Beta

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9
Q

Bacillus cereus: Gram stain

A

Large GPB; boxcar with spores

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10
Q

Bacillus cereus: clinical significance?

A
  • Reservoir: soil, straw & rice
  • Food poisoning
  • Opportunistic infections
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11
Q

Bacillus cereus: Important tests

A
  • Egg yolk agar POS
  • Growth on PEA
  • Lecithinase POS
  • Motility POS
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12
Q

Corynebacterium spp: Gram stain

A

Small GPB palisading, club shaped

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13
Q

Corynebacterium spp: clinical significance?

A

-Typically normal flora of skin and respiratory tract

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14
Q

Corynebacterium diphtheriae: clinical significance?

A
  • Diphtheria toxin
  • Diphtheric pseudomembrane
  • Specimen types: oropharynx and nasopharynx swabs
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15
Q

Corynebacterium diphtheriae: Important tests

A
  • Elek test positive
  • Grows on BAP
  • Loeffler for GS morph (methylene blue)
  • T/C TBA selective for thisbrownish/black colonies
  • Ferments glucose and maltose
  • Nitrate reduction positive
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16
Q

Corynebacterium jeikeium: Colony morphology

A

Gray white nonhemolytic; grows better on blood than choc(lipophilic)

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17
Q

Corynebacterium jeikeium: clinical significance?

A
  • Colonizes skin of hospital patients
  • Infections in immunocompromised hosts
  • Multi drug resistance seen
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18
Q

Corynebacterium jeikeium: Important tests

A

Resistant to P10 disk

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19
Q

Corynebacterium urealyticum: Colony morphology

A

White, pinpoint nonhemolytic and lipophilic

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20
Q

Corynebacterium urealyticum: clinical significance?

A

-urinary pathogen

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21
Q

Corynebacterium urealyticum: important tests

A

-Urease positive

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22
Q

Erysipelothrix rhusiopathiae: Colony morphology

A

Small colonies on BA, greenish coloration but not alpha hemolytic

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23
Q

Erysipelothrix rhusiopathiae: gram stain

A

Slender GPB, can be filamentous

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24
Q

Erysipelothrix rhusiopathiae: clinical significance?

A

Reservoir: Mammals, fish, birds

  • Contact with tissues of infected animals
  • Ingestion of contaminated meat
  • Mild localized cutaneous infection, usually on fingers and hands
  • Specimen types: biopsy, blood cultures
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25
Q

Erysipelothrix rhusiopathiae: important tests

A

H2S positive

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26
Q

Gardnerella vaginalis:Colony morphology

A

Pinpoint nonhemolytic

May take >24 hrs to grow

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27
Q

Gardnerella vaginalis:gram stain

A

Gram variable bacilli

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28
Q

Gardnerella vaginalis: clinical significance?

A

-Normal flora of female genital tract
-Bacterial vaginosis
-UTIs in men and women
Specimen: genital culture

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29
Q

Gardnerella vaginalis:important tests

A

-Wet prep: >20% clue cells
-Whiff test
-Grows best with increased CO2
V agar: hemolysis on HBT and purple yellow
Sodium Hippurate POS

30
Q

Lactobacillus sp.:Colony morphology

A

Alpha

31
Q

Lactobacillus sp.: gram stain

A

Long slender GPB

32
Q

Lactobacillus sp.: clinical significance?

A
  • Normal flora of female genital tract

- Specimens: urine culture

33
Q

Lactobacillus sp.: important tests

A

Some grow best anaerobically

34
Q

Listeria monocytogenes: Colony morphology

A

Looks similar to Group B strep, narrow “soft” zone of Beta

35
Q

Listeria monocytogenes: gram stain

A

GPB or short, plump coccobacilli

36
Q

Listeria monocytogenes: clinical significance?

A
  • Reservoir: soil, water, sewage, vegetation
  • Biofilm grows well at 4C
  • Colonized mothers can pass to fetus
  • Infection of CNS with high mortality rate
  • Never normal flora in genital and amniotic fluid specimens
37
Q

Listeria monocytogenes: important tests

A
  • Growth at 4C
  • Sodium Hippurate POS
  • CAMP test POS block type
  • Bile esculin POS
  • Motility POS: tumbling on wet mount and umbrella pattern at RT
38
Q

Rhodococcus spp.(especially equi): Colony morphology

A

Nonhemolytic, round, sometimes mucoid, often turns pink/orange/red after a few days

39
Q

Rhodococcus spp.(especially equi): gram stain

A

Diphtheroid like GS

40
Q

Rhodococcus spp.(especially equi): clinical significance?

A
  • Very diverse group

- Infections in immunocompromised patients

41
Q

Rhodococcus spp.(especially equi): important tests

A
  • Partially acid fast

- Needs Rapid ID systems

42
Q

Which species are catalase positive?

A

Bacillus
Corynebacterium
Listeria monocytogenes
Rhodococcus

43
Q

Which species are catalase negative?

A

Arcanobacterium
Erysipelothrix
Lactobacillus
Gardnerella vaginalis

44
Q

Which are beta hemolytic?

A

Arcanobacterium
Bacillus cereus
Listeria monocytogenes

45
Q

Which are lethicinase positive?

A

Arcanobacterium

Bacillus spp

46
Q

Which is -Typically normal flora of skin and respiratory tract?

A

Corynebacterium spp

47
Q

Which are normal flora of female genital tract?

A

Lactobacillus

Gardnerella vaginalis

48
Q

Which is H2S positive?

A

Erysipelothrix rhusiopathiae

49
Q

Which one: Small colonies on BA, greenish coloration but not alpha hemolytic?

A

Erysipelothrix rhusiopathiae

50
Q

filamentous gram stain?

A

Erysipelothrix rhusiopathiae

51
Q

which one may take >24 hrs to grow

A

Gardnerella vaginalis

52
Q

which one is Alpha hemolytic?

A

Lactobacillus sp.

53
Q

Some grow best anaerobically applies to ?

A

Lactobacillus sp.

54
Q

Which one has this gram stain: GPB or short, plump coccobacilli?

A

Listeria monocytogenes

55
Q

Biofilm grows well at 4C?

A

Listeria monocytogenes

56
Q

Colonized mothers can pass to fetus?

A

Listeria monocytogenes

57
Q

Never normal flora in genital and amniotic fluid specimens?

A

Listeria monocytogenes

58
Q

Diphtheroid like GS?

A

Rhodococcus spp.

59
Q

Partially acid fast?

A

Rhodococcus spp.

60
Q

Which are positive for motility?

A

Bacillus cereus

Listeria monocytogenes

61
Q

Which is positive for bile esculin?

A

Listeria monocytogenes

62
Q

Which is positive for CAMP test?

A

Listeria monocytogenes- block type

63
Q

Which is positive for Sodium hippurate?

A

Listeria monocytogenes

64
Q

Which is positive for urease?

A

Corynebacterium urealyticum

65
Q

Which is positive for nitrate reduction?

A

Corynebacterium diphtheriae

66
Q

Tumbling (wet) or umbrella pattern (RT) motility?

A

Listeria monocytogenes

67
Q

3 forms of Bacillus anthracis?

A

Cutaneous anthrax
Pulmonary anthrax
Gastrointestinal anthrax

68
Q

Most common form of Bacillus anthracis?

A

Cutaneous anthrax

69
Q

Which form of Bacillus anthracis is associated with Woolsorter’s Disease

A

Pulmonary anthrax

70
Q

Principle of egg yolk agar?

A

Lethicinasebreak down lethicin into insoluble diglyceridewhite opaque zone around colony

71
Q

Principle of ELIK test?

A

Immunodiffusion: precipitation lines form at 45 degree angle if positive

Microbiology (11 decks)

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