Unit 4 Flashcards
The two stages of protein synthesis in order
transcription and translation
DNA to mRNA
Transcription
mRNA to protein
Translation
Steps of Transcription
- DNA unzips (with RNA polymerase) so it can be used as a template to make mRNA
- RNA polymerase adds nucleotides (using complementary base pairing) to the mRNA
- mRNA breaks off and the DNA zips back together, mRNA leaves the nucleus to look for a ribosome
Steps of Translation
1.The ribosome reads mRNA and finds the start codon (AUG).
- A tRNA molecule with a complementary anticodon drops off an amino acid.
- This continues at each mRNA codon, with the tRNA complimentary base pairing and dropping of its amino acid. The amino acids bond together making a polypeptide chain or protein.
- This process repeats over and over until the ribosome reaches a STOP codon.
three-nucleotide sequence on messenger RNA that codes for a single amino acid
codon
AUG (methionine)
start codon
UAA, UGA, UAG
stop codons are
Gene expression
DNA -> mRNA -> Protein -> trait
Site of replication in eukaryotes
nucleus
The site of translation
cytoplasm and ribosomes
point mutation in which one nitrogeneous base (nucleotide) is changed to another
substitution mutation
a mutation in which a nucleotide is added to a DNA sequence in a gene
insertion mutation
a mutation in which a nucleotide is removed from a DNA sequence in a gene
deletion mutation
point mutation
a mutation affecting only one or very few nucleotides in a gene sequence.