UNIT 2 - B 2.2 - Organelles and Compartmentalization Flashcards
What is cell compartmentalization?
The isolation of reactions so they don’t interfere with one another
What is the result of cell compartmentalization?
cells work much more efficiently
What is reductionism?
Studying cells by breaking them down into individual components
What does fractionation refer to in biochemical fractionation?
the separation and isolation of spedivid chemicals/structures so detailed research can be carried out
What is another word for cell fractionation?
Centrifugation
What does centrifugation allow?
the extraction of organelles from the cell
What is the process of cell fractionation?
cells are mixed in a tube with substances that break down the cell membranes, the sample is spun in an ultracentrifuge to isolate the different components, larger/heavier components go to the bottom of the tube and seperate at lower speeds
What technique is effective in isolating pure substances such as amino acids, proteins, carbohydrates, and plant pigmants?
chromatography
What is the process of chromatography?
A mixture of molecules is placed in a separating medium, and the molecules seperate depending on size and speed they travel through the medium
How does gel electrophoresis separate molecules?
by passing them through a gel using an electric charge
What are molecules separated based on with gel electrophoresis?
size and charge
Why are barriers around organelles important?
It allows the chemical reactions in an organelle to not be interfered with from the rest of the cell
What components of cells are not organelles?
cell wall, cytoskeleton, and cytoplasm
What components of cells are considered organelles?
nucleus, vesicles, ribosomes, plasma membrane, cilia/flagella, golgi apparatus, mitochondria, chloroplasts, and lysosomes
What are the processes responsible for the production of proteins called?
transcription and translation
What is involved in transcription?
a DNA strand acts as a template for the formation of mRNA
What is involved in translation?
Ribosomes use the code from the mRNA to produce polypeptide chains
Where does transcription occur?
the nucleus of eukaryotic cells
Where does translation occur?
in the cytoplasm
What does the seperation of transcription and translation allow?
post-transcriptional modifications of mRNA to occur in the nucleus before translation occurs in the cytoplasm
How does the transcription/translation process differ in prokayotic cells?
The two processes are not isolated so mRNA can come into contact with ribosomes and start translation immediately without any modifications
What does the number and size of organelles depend on?
the overall function of the cell in which they occur
What do acinar cells specialize in?
The secretion of digestive enzymes
Which organelles are greatly enlarged in acinar cells?
the ER, golgi apparatus and granule storage compartments
What does keeping reactions separate in different parts of the cell result in?
The metabolites and enzymes for each process and be concentrated in a particular area
What does the metabolites and enzymes being concentrated in a particular area allow?
That pathways run smoothly, can be easily controlled, and do not interfere with one another
What do lysosomes help do?
breakdown wastes and cellular components that need to be replaced
What would happen if the enzymes involved with the waste breakdown in lysosomes weren’t isolated by a membrane?
They could cause severe damage to the cell
What is often the result of endocytosis?
a phagocytic vacuole
What happens after the phagocytic vacuole comes into contact with a lysosome?
inactivation and digestion of the threatening contents of the vacuole
What does phagocytosis help defend the cell against?
invading phathogens
What must happen due to the cell having organelles carrying out one specific function?
They have to develop a means of integrating each separate function
How are some of the separate functions integrated?
Some organelles are connected in a functional series allowing chemical pathways to take place
Why have membrane pumps and carriers evolved?
So that the products of one organelle can enter another and important cell reactions can occur
What is cell respiration?
The process by which glucose is used to produce ATP which then provides energy needed for cell reactions
Where does respiration (the complex chemical reactions) mostly occur?
In the mitochondrion
Why do all organisms carry out respiration?
All organisms must be able to produce ATP for energy
What is the outer mitochondrial membrane?
A membrane that separates the contents of the mitochondrion from the rest of the cell